Method for improving Anthurium andraeanum conversion efficiency

A technology of transformation efficiency and anthurium, which is applied in biochemical equipment and methods, horticultural methods, botanical equipment and methods, etc., can solve the problems of genetic transformation of anthurium stem segments, so as to improve the positive rate of plant transformation and reduce the Test cost, effect of inhibiting Agrobacterium contamination

Active Publication Date: 2021-03-19
山东和正生态农业开发有限公司
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Problems solved by technology

After literature search, there is no related report on the genetic transformation of Anthurium stem segments

Method used

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Embodiment Construction

[0020] In order to clearly illustrate the technical features of the present solution, the present application will be described in detail below through specific implementation modes.

[0021] A kind of method of improving Anthurium transformation efficiency of the present invention, general steps are as follows:

[0022] S1. Acceptor material preparation;

[0023] Select 20-25d sterile Anthurium seedlings, cut the stems with axillary buds and pre-cultivate them as recipient materials; the pre-cultivation time is not less than 2 days;

[0024] S2. Bacterial solution preparation;

[0025] Use the plant expression vector pBI 121 with GFP gene, connect the chrysanthemum Flavanone3-hydroxylase gene by enzyme digestion, and then use the freeze-thaw method to introduce Agrobacterium E.coli DH5a to obtain the Agrobacterium with the plant expression vector pBI121-GFP-Flavanone 3-hydroxylase E.coli DH5a bacterial liquid;

[0026] Aspirate the bacterial liquid, transfer it to the LB l...

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Abstract

The invention discloses a method for improving Anthurium andraeanum conversion efficiency. The method comprises a process that through a bacterial solution, an exogenous gene is imported into a receptor material of Anthurium andraeanum, wherein the receptor material is from a stem segment with an axillary bud. The method adopts the stem segment with the axillary bud as the receptor material, a positive plant can be quickly obtained, and culture efficiency is greatly improved.

Description

technical field [0001] The present application relates to a method for improving the transformation efficiency of anthurium. Background technique [0002] Anthurium andraeanum, also known as anthurium, is a perennial evergreen herb of Araceae. The stem nodes are short, the leaves grow from the base, green, leathery, entire, oblong-heart-shaped or ovate-heart-shaped. The petioles are slender, the spathes are flat, leathery and waxy, orange-red or scarlet, and the spadix is ​​yellow. It can bloom continuously all year round. It is native to tropical rainforest areas such as Costa Rica and Colombia. Often epiphytic on trees, sometimes epiphytic on rocks or directly on the ground, prefers warm, humid, semi-shady environment, avoid direct sunlight. Anthurium has beautiful flowers and leaves, long flowering period, and is a high-quality cut flower material. Red anthurium is used as the main material, with small chrysanthemum, Xiacao, Yanshan ginger, and variable leaf wood vase...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82C12N15/53C12N15/65A01H4/00A01H5/00A01H6/10
CPCC12N15/8205C12N15/65C12N9/0071A01H4/008C12Y114/11009Y02P60/20
Inventor 刘会君程曼马志范士猛
Owner 山东和正生态农业开发有限公司
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