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Cathodic electric fermentation method for preparing hydrogen and butanol by electron transfer mediator enhanced clostridium beijerinckii fermentation

An electron transfer mediator, the technology of Clostridium beijerinckii, applied in biochemical equipment and methods, microorganism-based methods, fermentation, etc., can solve the failure of acid-producing phase to solvent-producing phase transition, butanol yield, low yield, The problem of high proportion of acetone can increase the synthesis of ATP and cellular substances, increase the yield and yield, and improve the composition.

Active Publication Date: 2021-03-16
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention provides an electron transfer mediator in order to solve the problems of the existing Clostridium beijerinckii in the butanol fermentation process, such as low butanol yield and yield, failure of phase transition from acid-producing phase to solvent-producing phase, and high proportion of acetone in the solvent Cathodic electrofermentation method for strengthening the fermentation of Clostridium beijerinckii to produce hydrogen and butanol

Method used

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  • Cathodic electric fermentation method for preparing hydrogen and butanol by electron transfer mediator enhanced clostridium beijerinckii fermentation
  • Cathodic electric fermentation method for preparing hydrogen and butanol by electron transfer mediator enhanced clostridium beijerinckii fermentation
  • Cathodic electric fermentation method for preparing hydrogen and butanol by electron transfer mediator enhanced clostridium beijerinckii fermentation

Examples

Experimental program
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Effect test

Embodiment 1

[0027]An anaerobic mixed flora fermentation straw produced in a cathode electrical fermentation system (Cef + NR) added to an exogenous electron transfer medium. The specific procedure is as follows:

[0028](1) The fermentation reactor is carried out in a fermentation reactor with a valid volume of 100 ml of the double chamber H-type reactor, wherein the fermentation is carried out in the cathode chamber, and there is an equivalent of 200 mmol / L of ferricinide in the anode chamber, as electrons in the electric fermentation system. Donor. The graphite felt and the anode fixed to the top of the titanium wire at a diameter of 3 mm, and the cathode and an anode are fixed to the top of the polar chamber are fixed. The anode reference electrode is Ag / AGCL (196mv vs she), which is fixed by the cathode chamber side, and the front end is placed on the front end of the fermentation liquid.figure 1 Indicated.

[0029](2) Adjust the anaerobic fermentation medium that is configured to add a ferme...

Embodiment 2

[0042](1) Fermentation straw produced in anaerobic hybrid flora fermentation straw in the cathode electrical fermentation system (Cef + NR) added exogenous electron transfer mediators. Different from Example 1, the fermentation substrate was glucose and butyric acid, 1L anaerobic fermentation medium added fermented substrate glucose was 30 g, 1L anaerobic fermentation medium added fermented substrate butyric acid was 3 g, and the remaining operation Similarly to Example 1.

[0043](2) Anaerobic mixed flora fermentation straw produced in the system (OC) without application potential. At different comparative examples 1, the fermentation substrate is glucose and butyric acid, 1L anaerobic fermentation medium to add fermented substrate glucose to 30 g, 1L anaerobic fermentation medium to add fermented substrate butyric acid to 3 g, and operate The same as the contrast 1.

[0044]The fermentation index data obtained (1) and (2), as shown in Table 1 below.

[0045]Table 1 Butanol fermentation per...

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Abstract

The invention discloses a cathodic electric fermentation method for preparing hydrogen and butanol by electron transfer mediator enhanced clostridium beijerinckii fermentation, and belongs to the technical field of clean energy production through microbial fermentation. The method solves the problems of low butanol output and yield, failure in transformation from an acid producing phase to a solvent producing phase and relatively high acetone proportion in a solvent in a butanol fermentation process for existing clostridium beijerinckii. According to the invention, the butanol is prepared by clostridium beijerinckii fermentation in a cathodic electric fermentation system; and by introducing electrodes and adding exogenous electron transfer mediator neutral red (NR), a fermentation environment can be controlled and optimized, original NAD+ / NADH balance of cells can be changed, shunting of different ways in microbial metabolism can be significantly optimized, more carbon and electrons ina microbial metabolism path are induced to flow to a butanol synthesis path, the output and yield of the biobutanol are improved, and more hydrogen is generated at the same time.

Description

Technical field[0001]The present invention relates to a cathode electrical fermentation method for electron transfer medium to enhance Bastoria fermentation, a cathode electrical fermentation method of hydrogen gas and butanol, belonging to microbial fermentation production, cleaning energy technology.Background technique[0002]Renewable biomass, reserves, can be converted into a variety of high-efficiency energy sources, such as biological ethanol, biodiesel, biocyanol, and biogas, etc., can be important in response to global climate change, energy supply and demand contradictions, and protecting the ecological environment in response to global climate change, energy supply and demand. effect. Under the promotion of national energy strategies, biological ethanol has been promoted. Compared with ethanol, biocyanol has the advantages of high energy, good mixability, low volatility and minor corrosive, is a very potential new biofuel. And the energy density of butanol has a lower satur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P3/00C12P7/16C12N13/00C12R1/145
CPCC12P3/00C12P7/16C12N13/00Y02E50/10
Inventor 李建政张亚非孟佳王鑫
Owner HARBIN INST OF TECH
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