Hematuria-reducing lactic acid bacteria strain and screening method and application thereof in preparation of functional yoghourt

A screening method and technology of lactic acid bacteria, applied in the direction of bacteria, applications, and lactobacilli used in food preparation, can solve the problems of lack of special functional factors and single function of yogurt, and achieve good acid and bile salt resistance and antibacterial properties, good The effect of hydrophobicity

Active Publication Date: 2021-03-09
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although yogurt is rich in nutrients such as protein, the functions of yogurt currently available on the market are relatively single, but lack special functional factors.

Method used

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  • Hematuria-reducing lactic acid bacteria strain and screening method and application thereof in preparation of functional yoghourt
  • Hematuria-reducing lactic acid bacteria strain and screening method and application thereof in preparation of functional yoghourt

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Embodiment 1

[0044] A strain of lactic acid bacteria that efficiently degrades purine nucleosides and lowers blood uric acid. The lactic acid bacteria is named as Lactobacillus brevis (Lactobacillus brevis) The PDD-5 strain was deposited in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures on August 31, 2020. The preservation number is CGMCC No. 20573, and the preservation address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, China Institute of Microbiology, Academy of Sciences.

Embodiment 2

[0046] The above-mentioned embodiment 1 efficiently degrades purine nucleosides and the screening method for lactic acid bacteria that can reduce blood uric acid comprises the following steps:

[0047] (1) Preliminary screening and isolation and purification of lactic acid bacteria

[0048] Select fresh milk, Chinese pickles, and Chinese Xinjiang milk pimples as samples to dilute, spread on the MRS solid medium plate containing bromocresol purple, let it stand, and cultivate it at 37-43°C for 36-48h, and select yellow colonies A single colony with typical characteristics of lactic acid bacteria is preliminarily determined as lactic acid bacteria; the initially determined colony is repeatedly streaked several times on the MRS solid medium plate, so as to further purify until a single colony with the same shape appears, Save the strains for future use. The preparation method of the MRS solid medium containing bromocresol purple is as follows: dissolve 52.24g of MRS broth, 0.04g...

Embodiment 3

[0053] Lactobacillus brevis (Lactobacillus brevis) The growth curve of the PDD-5 strain in the culture medium: the activated Lactobacillus brevis (Lactobacillus brevis) The PDD-5 strain was inoculated into MRS medium with an inoculum of 1% by volume and cultured at 37-43°C. Determination of bacterial cell OD at different growth times 600 value. Lactobacillus brevis (Lactobacillus brevis) The PDD-5 strain is in the logarithmic growth phase at 5-22 hours. During this period, the growth rate constant R is the largest, the growth rate of the bacteria is the fastest, and a large amount of lactic acid is produced (the pH of the fermentation broth is maintained at about 4). After 22 hours, the bacteria grow into the stabilizer, the death and growth of the bacteria reach a dynamic balance, and the growth rate constant R is basically zero. At this time, the growth rate of the bacteria slows down and tends to be stable. suppressed. In addition, the fermentation broth OD within 7...

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Abstract

The invention discloses a hematuria-reducing lactic acid bacteria strain and a screening method and application thereof in preparation of functional yoghourt. The strain is characterized in that the lactic acid bacteria strain is classified and named as Lactobacillus brevis PDD-5 strain, is preserved in China General Microbiological Culture Collection Center on August 31, 2020, and has a preservation number of CGMCC No. 20573. The screening method comprises the following steps of (1), selecting traditional fermented food pickles as samples, and carrying out preliminary screening and separationpurification on lactic acid bacteria; and (2), obtaining strains with negative catalase activity and positive gram staining, then culturing and collecting thalli, re-suspending the thalli in an inosine guanosine solution and culturing, and then selecting lactic acid bacteria with the highest degradation rate on inosine or guanosine. The method has the advantages of oxidation resistance, inflammation resistance, immunoregulation, blood uric acid reduction, capability of relieving and treating hyperuricemia and the like.

Description

technical field [0001] The invention relates to a strain of lactic acid bacteria capable of efficiently degrading purine nucleosides, in particular to a strain of lactic acid bacteria capable of reducing uric acid, a screening method thereof, and the preparation and application of functional yoghurt. Background technique [0002] Purine is an important base in organisms and is an organic compound. It is formed by combining pyrimidine ring and imidazole ring. Purine nucleotides and pyrimidine nucleotides are the main components of cells and the basic units of DNA and RNA, and play an important role in cell structure, metabolism, energy and function regulation. Purines in the human body mainly include adenine, guanine, hypoxanthine and xanthine, and purines are metabolized in the human body to form uric acid. When the uric acid in the human body is too high, it will cause gout. Gout is a group of heterogeneous diseases with increased blood uric acid caused by purine metaboli...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02A61K35/744A61P31/04A61P19/06A23C9/123C12R1/24
CPCC12N1/20C12N1/02A23C9/1236A23C9/1234A61K35/744A61P31/04A61P19/06A23V2400/121A23V2400/249Y02A50/30
Inventor 潘道东徐钰吴振曾小群
Owner NINGBO UNIV
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