Preparation method of low-pungency antioxidant ginger essential oil byproduct beverage
A technology for ginger essential oil and by-products, which is applied in the field of ginger essential oil and by-product reuse, can solve the problems of low antioxidant property, unfriendly, irritating and spicy taste, etc., and achieves the effects of improving antioxidant capacity, good drinking taste and good flavor.
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Embodiment 1
[0026] This embodiment provides a preparation method of a low-pungent anti-oxidation ginger essential oil by-product beverage, the preparation method is as follows:
[0027] 1. Take 0.5g of freeze-dried Lactobacillus acidophilus powder and dissolve it in 0.2mL MRS liquid medium, inoculate it on 4 MRS medium plates, and incubate at a constant temperature of 37±1°C for 24 hours. Select a single colony with good shape and vigorous growth from the plate, inoculate it in 8 mL of MRS liquid medium for recovery, and culture it statically at 37±1°C until the solution appears turbid to the naked eye to obtain the recovery bacteria solution. Then the bacterial solution was mixed with 60% glycerol at a ratio of 1:1 (v:v), and then stored in a freezer.
[0028] 2. Prepare 50mL of MRS liquid medium, add 0.5mL of resuscitated bacteria solution to it, and expand it to the logarithmic growth phase in a constant temperature incubator at 37±1°C. The liquid medium has turbidity visible to the na...
Embodiment 2
[0031] This embodiment provides a preparation method of a low-pungent anti-oxidation ginger essential oil by-product beverage, the preparation method is as follows:
[0032] 1. Take 2g of freeze-dried Lactobacillus acidophilus powder and dissolve it in 0.3mL MRS liquid medium, inoculate it on 2 MRS medium plates, and incubate at a constant temperature of 37±1°C for 72 hours. Select a single colony with good shape and vigorous growth from the plate, inoculate it in 10 mL of MRS liquid medium for recovery, and culture it statically at 37±1°C until the solution appears turbid to the naked eye to obtain the recovery bacteria solution. Then the bacterial solution was mixed with 60% glycerol at a ratio of 1:1 (v:v), and then stored in a freezer.
[0033] 2. Prepare 50mL of MRS liquid medium, add 1mL of resuscitated bacteria liquid to it, and expand the culture in a constant temperature incubator at 37±1°C to the logarithmic growth phase. The liquid medium has turbidity visible to th...
Embodiment 3
[0036] This embodiment provides a preparation method of a low-pungent anti-oxidation ginger essential oil by-product beverage, the preparation method is as follows:
[0037] 1. Take 0.5g of freeze-dried Lactobacillus acidophilus powder and dissolve it in 0.3mL MRS liquid medium, inoculate on 3 MRS medium plates, and incubate at a constant temperature of 37±1°C for 24 hours. Select a single colony with good shape and vigorous growth from the plate, inoculate it in 10 mL of MRS liquid medium for recovery, and culture it statically at 37±1°C until the solution appears turbid to the naked eye to obtain the recovery bacteria solution. Then the bacterial solution was mixed with 60% glycerol at a ratio of 1:1 (v:v), and then stored in a freezer.
[0038]2. Prepare 50mL of MRS liquid medium, add 3mL of resuscitated bacteria solution to it, and expand the culture in a constant temperature incubator at 37±1°C to the logarithmic growth phase. The liquid medium has turbidity visible to th...
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