DNA-functionalized copper oxide nanozyme, preparation method and application thereof
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A nano-enzyme and copper oxide technology, applied in the fields of materials, nano-carriers and nano-biology, achieves the effects of mild reaction conditions, simple operation, and increased ROS levels
Active Publication Date: 2022-02-15
湖南修悟生物科技有限公司
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However, based on the law of DNA adsorption, the design of DNA-functionalized nanozymes has not been reported in the targeted therapy of tumors.
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Embodiment 1
[0046]A polarization-controlled DNA functionalized CuO nanozyme, including di-DNA and CuO nanozyme, di-DNA is a two-block DNA with (TC) at one end 12 DNA (SEQ ID NO.1: TCTCTCTCTCTCTCTCTCTCTCTC), the other end is FAM-A 15 (SEQID NO.2: AAAAAAAAAAAAAAA), di-DNA is adsorbed on CuO nanozyme through the DNA sequence composed of TC bases.
[0047] A method for preparing the polarization-controlled DNA functionalized CuO nanozyme of the present invention, comprising the following steps:
[0048] (1) Disperse 1 μM di-DNA in 10 mM HEPES buffer (pH7.6, 150 mM NaCl) to obtain a dispersion;
[0049] (2) Adsorb 100 μg / mL CuO nanozyme and dispersion liquid at 4°C for 2 h to obtain polarization-controlled DNA functionalized CuO nanozyme;
[0050] (3) Add 1% FBS inactivated by high temperature, mix well by ultrasonic, and continue to incubate for 1 h.
[0051] (4) Centrifuge at 4° C., 16000 rpm for 30 min, remove the supernatant, wash the precipitate, disperse in HEPES buffer (10 mM, pH 7.6...
Embodiment 2
[0053] A polarization-controlled DNA functionalized CuO nanozyme, including di-DNA and CuO nanozyme, di-DNA is a two-block DNA with (TC) at one end 12 DNA (SEQ ID NO.1: TCTCTCTCTCTCTCTCTCTCTCTC), the other end is FAM-T 15 (SEQID NO.3: TTTTTTTTTTTTTTT), di-DNA is adsorbed on CuO nanozyme through the DNA sequence composed of TC bases.
[0054] Its preparation method is consistent with embodiment 1.
Embodiment 3
[0056] A polarization-controlled DNA functionalized CuO nanozyme, including di-DNA and CuO nanozyme, di-DNA is a two-block DNA with (TC) at one end 12 DNA (SEQ ID NO.1: TCTCTCTCTCTCTCTCTCTCTCTC), the other end is FAM-C 15 (SEQID NO.4: CCCCCCCCCCCCCCC), di-DNA is adsorbed on CuO nanozyme through the DNA sequence composed of TC bases.
[0057] Its preparation method is consistent with embodiment 1.
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Abstract
The present invention provides a DNA functionalized copper oxide nanozyme and its preparation method and application. The DNA functionalized copper oxide nanozyme includes di-DNA and CuO nanozyme, one end of di-DNA is a DNA sequence composed of TC bases, and the other One end is a DNA sequence composed of nucleosides, and di-DNA is adsorbed on CuO nanozyme through the DNA sequence composed of TC bases. The DNA functionalized copper oxide nanozyme of the present invention is designed based on the adsorption mechanism of DNA on the surface of the copper oxide nanozyme, can be polarized adsorbed on the surface of the nanozyme, and endows tumor targeting function. The preparation method is as follows: dispersing di-DNA into a buffer solution to obtain a dispersion liquid; mixing the dispersion liquid with CuO nanozyme for adsorption. The preparation method of the invention has the advantages of simple operation, low cost and mild reaction conditions, can be used for tumor-targeted modification of nanozymes, and has broad application prospects in the fields of disease treatment and the like.
Description
technical field [0001] The invention relates to the fields of materials, nanocarriers and nanobiotechnology, in particular to a DNA functionalized copper oxide nanozyme and its preparation method and application. Background technique [0002] The tumor microenvironment (Tumor microenvironment, TME) is an abnormal microenvironment with low oxygen, slightly acidic and high levels of H 2 o 2 specialty. Taking advantage of the significant differences between the TME and normal tissues, various anti-tumor therapeutic strategies can be tailored. For example, anaerobic Salmonella can reproduce in the TME in the hypoxic region, and this feature can be used to construct a tumor-targeted drug delivery system with Salmonella; nanozymes assembled from acid-sensitive polymers can respond to acidic TME to achieve tumor Targeting; or using the high level of hydrogen peroxide in the TME to generate singlet oxygen and enhance the combined anti-tumor efficiency. [0003] Nanozyme is a kin...
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