Rapid detection system and method

A detection system and detection method technology, applied in biochemical equipment and methods, measurement devices, biochemical instruments, etc., can solve the problems of long detection time, inability to detect drug resistance mechanisms, and inability to meet the timeliness requirements of clinical applications, etc.

Pending Publication Date: 2021-02-26
SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, based on drug resistance gene analysis methods, it is not yet possible to detect new drug resistance mechanisms, and it is not possible to determine whether the strains containing drug resistance genes are in a state of drug resistance.
Existing detection methods and instruments have problems such as long time-consuming detection, inability to detect unknown pathogenic bacteria, etc., and cannot meet the timeliness requirements of clinical applications. This application provides a rapid detection system and method

Method used

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Examples

Experimental program
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Embodiment

[0092] This application involves two parts, the system and the method, so some implementations are introduced.

[0093] 1. Prepare the chip, including the following steps:

[0094] a. Photolithographic preparation of a microfluidic chip template based on a silicon wafer substrate: the template structure includes a plurality of microfluidic channels, and each microfluidic channel is composed of a connected sample inlet, a main channel, a bacteria capture channel, and a sample outlet. The main channel is 100 microns wide and 20 microns high, for the continuous flow of medium liquid; the bacteria capture channel is 25, 1, and 1 micron in length, width and height, and is arranged side by side on both sides of the main channel to form semi-closed channels for capturing single bacteria. The partial enlarged schematic diagram of the microfluidic chip template is shown in Figure 4 shown;

[0095] b. Preparation of polydimethylsiloxane (PDMS)-based microfluidic chip: PDMS prepolyme...

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Abstract

The invention belongs to the technical field of biological detection, and particularly relates to a rapid detection system and method. Existing detection methods and instruments have the problems of long detection time, failure to detect unknown pathogenic bacteria and the like, and cannot meet the requirement of clinical application for timeliness. The invention provides a rapid detection system.The system comprises a bacteria culture and capture subsystem, an imaging subsystem, an image processing subsystem, a data analysis subsystem and a terminal, wherein the terminal is connected with the bacteria culture and capture subsystem, the imaging subsystem, the image processing subsystem and the data analysis subsystem, and the image processing subsystem is connected with the data analysissubsystem. By means of the rapid detection system and method, the function of quickly detecting the drug resistance of various clinical pathogenic bacteria within 2 hours can be realized.

Description

technical field [0001] The application belongs to the technical field of biological detection, and in particular relates to a rapid detection system and method. Background technique [0002] Bacterial drug resistance has become a worldwide problem, which refers to the resistance of bacteria to antibacterial drugs. The emergence of drug resistance will significantly reduce the therapeutic effect of drugs. Bacterial drug resistance is an inevitable natural evolutionary process, but the overuse of antibiotics has greatly accelerated this process in recent years, posing great challenges to human buffer time and coping strategies. Rapid determination of bacterial susceptibility to antibiotics is the premise and key to provide timely and reasonable guidance for clinical treatment. The detection of drug resistance in clinical treatment includes the steps of sample collection from infected patients, in vitro isolation and culture, and antibiotic sensitivity detection. According t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/38C12M1/34C12M1/00G01N21/84
CPCC12M23/16C12M41/12C12M41/46G01N21/84
Inventor 黄术强李思宏刘陈立傅雄飞于跃
Owner SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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