A kind of aseptic seedling cultivation method of Aconitum radix

A cultivation method and technology for sterile seedlings, applied in the biological field, can solve the problems of reducing the browning rate of callus, the survival rate of seedlings, and the reduction of browning rate, and achieve the effects of good application prospect, strong growth and improvement of survival rate.

Active Publication Date: 2022-04-08
INNER MONGOLIA M GRASS ECOLOGY & ENVIROMENT GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to make up for the deficiencies in the prior art, the invention provides a method for cultivating aseptic seedlings of Aconitum radix, through the method of the invention, the function of effectively reducing the browning rate of callus and high survival rate of seedlings is realized, solving the problem of traditional The problem of the low survival rate of seedlings is dealt with in sand storage, and the survival rate of seedlings is improved; the pollution rate of seedlings is reduced to below 3%, the browning rate is reduced to 0%, and the number of rooted bars reaches 45-60 roots / bottle

Method used

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  • A kind of aseptic seedling cultivation method of Aconitum radix
  • A kind of aseptic seedling cultivation method of Aconitum radix
  • A kind of aseptic seedling cultivation method of Aconitum radix

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Embodiment 1

[0055] A method for cultivating aconitum aconitum aseptic seedlings, specifically comprising the following steps;

[0056] S1: Select Aconitum aconitifolia branches with soft and tender skin as explants, and the size of the explants is 3-5mm;

[0057] S2: Disinfect the selected explants. The specific method is: first soak with agricultural streptomycin for 4 minutes, then rinse with sterile water for 4 times, each time for 50 seconds, then soak in 0.1% mercuric solution for 7 minutes, and then use Rinse the sterile water device 4 times;

[0058] S3: placing the sterilized explants into the primary culture medium for inducing culture to form callus;

[0059] The primary medium is: GS+6-BA 1mg / L+NAA 0.45mg / L+AC 5mg / L+KT 0.05mg / L;

[0060] The conditions for the first-generation induction culture are that the culture temperature is 20°C, the light intensity is 2500Lx, and the light is not supplemented at night except for the natural light during the day, and the culture period ...

Embodiment 2

[0068] A method for cultivating Aconitum aconitum aseptic seedlings, specifically comprising the following steps:

[0069] S1: Select Aconitum aconitifolia branches with soft and tender skin as explants, and the size of the explants is 3-5mm;

[0070] S2: Disinfect the selected explants. The specific method is: first soak with agricultural streptomycin for 5 minutes, then rinse with sterile water for 5 times, each time for 60 seconds, then soak in 0.1% mercuric solution for 8 minutes, and then use Rinse the sterile water device 5 times;

[0071] S3: placing the sterilized explants into the primary culture medium for inducing culture to form callus;

[0072] The primary medium is: GS+6-BA 1mg / L+NAA 0.5mg / L+AC 5mg / L+KT 0.05mg / L;

[0073] The conditions for the first-generation induction culture are culture temperature 22°C, light intensity 3000Lx, no light supplement at night except natural light during the day, and the culture period is 22 days;

[0074] S4: Put the induced ...

Embodiment 3

[0081] A method for cultivating aconitum aconitum aseptic seedlings, specifically comprising the following steps;

[0082] S1: Select Aconitum aconitifolia branches with soft and tender skin as explants, and the size of the explants is 3-5mm;

[0083] S2: Disinfect the selected explants. The specific method is: first soak them with agricultural streptomycin for 4-6 minutes, then rinse them with a sterile water device for 6 times, each time for 70 seconds, and then soak them in 0.1% mercuric solution for 9 minutes. Rinse 6 times with a sterile water device;

[0084] S3: placing the sterilized explants into the primary culture medium for inducing culture to form callus;

[0085] The primary medium is: GS+6-BA 1mg / L+NAA 0.55mg / L+AC 5mg / L+KT 0.05mg / L;

[0086] The conditions for the first-generation induction culture are culture temperature 23°C, light intensity 5000Lx, no light supplement at night except natural light during the day, and the culture period is 24 days;

[0087]...

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Abstract

The invention discloses a method for cultivating aseptic seedlings of Aconitum aconitum, which specifically comprises the following steps: S1: selecting Aconitum aconitifolia branches with soft and tender skins as explants, and the size of the explants is 3-5 mm; S2: explanting the selected explants For disinfection, soak in agricultural streptomycin for 4-6min, then rinse with sterile water for 4-6 times, 50-70S each time, then soak in 0.1% mercuric solution for 7-9min, and then rinse with sterile water Rinse the device 4-6 times; S3: put the sterilized explants into the primary culture medium to induce culture to form callus; through the cultivation method of the present invention, the browning rate of callus and the survival of seedlings can be effectively reduced The function of high efficiency solves the problem of low survival rate of seedlings in traditional sand storage treatment and improves the survival rate of seedlings.

Description

technical field [0001] The invention relates to the field of biology, in particular to a method for cultivating aseptic seedlings of Aconitum aconitum. Background technique [0002] Aconitum, the name of traditional Chinese medicine; it is the dry tuberous root of Aconitum aconitum of the Ranunculaceae plant; excavated when the stems and leaves wither in autumn, remove fibrous roots and sediment, and dry; cultivate means to cultivate young organisms to make them grow; used for wind-cold-damp arthralgia , joint pain, confidant cold pain, cold colic pain and anesthesia pain relief; used to treat cold-damp blood stagnation in the meridian, limb muscle spasm pain, joint flexion and extension: same use with Chuanwu, earthworm, frankincense, etc.; often used as anesthesia Painkillers are mostly used together with raw products and raw Aconitum, and are compatible with sheep's weed and turmeric. [0003] Sand storage treatment is required before the germination of Aconitum aconitum...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 马怀林贾振宇张跃华高俊刚刘亚玲屈璐璐刘思泱高旭王乌恩巴图
Owner INNER MONGOLIA M GRASS ECOLOGY & ENVIROMENT GRP CO LTD
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