Method for establishing Caihong poplar regeneration system by taking leaves as explants
A technology of explants and poplar, applied in plant regeneration, botanical equipment and methods, horticultural methods, etc., can solve the problems of high differentiation rate, low pollution rate, difficulty in establishing the first-generation sterile line, etc., and achieve high-efficiency production Effect
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Embodiment 1
[0032] A method for establishing a poplar regeneration system with leaves as explants, comprising:
[0033] 1. Selection and disinfection of explants: at the end of June, the selected surface area was 5cm 2 Left and right young leaves, shake the detergent for 10 minutes, and rinse with running water for 30 minutes. Shake with 75% alcohol for 1 minute, then shake with 0.1% mercuric chloride for 12 minutes, wash with sterile water 6 times to remove residual disinfectant. A total of 100 explants were processed;
[0034] 2. Induction of adventitious buds: cut off the edge of the detoxified leaf explants, and retain an area of about 3cm 2 , the explants were inoculated in the induction medium MS+6-BA 1mg / L +NAA0.2 mg / L for adventitious bud induction, the culture temperature was controlled at 26°C, and cultured for 25 days, the culture conditions were: light time 14h / day, the light intensity was 1600lx; after 25 days, the explant contamination rate was 50%, and the induction r...
Embodiment 2
[0039] A method for establishing a poplar regeneration system with leaves as explants, comprising:
[0040] 1. Selection and disinfection of explants: at the end of June, the selected surface area was 5cm 2 Left and right young leaves, shake the detergent for 10 minutes, and rinse with running water for 30 minutes. Shake with 75% alcohol for 1 minute, then shake with 0.1% mercuric chloride for 12 minutes, wash with sterile water 6 times to remove residual disinfectant. A total of 100 explants were processed;
[0041] 2. Induction of adventitious buds: cut off the edge of the detoxified leaf explants, and retain an area of about 3cm 2 , the explants were inoculated in the induction medium MS+6-BA 1mg / L +NAA0.2 mg / L for adventitious bud induction, the culture temperature was controlled at 26°C, and the red light was irradiated for 1 day, and the red light had a wavelength of 720nm The electromagnetic radiation, the light intensity is 4800lx; Then continue to cultivate for 2...
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