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Preparation method and application of gold nanocage

A gold nanocage and mother solution technology, applied in nanotechnology, nanotechnology, nanomedicine, etc., can solve the problems of secondary transfection, cytokine storm, poor penetration, etc., achieve good penetration and simple operation process , highly specific effects

Active Publication Date: 2021-02-09
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In view of the above problems, the present invention provides a preparation method and application of gold nanocages, which mainly solves the problem that the prior art is prone to cause cytokine storm, requires secondary transfection, has poor penetrating power, and cannot be applied to different CAR-T markers And other issues

Method used

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  • Preparation method and application of gold nanocage
  • Preparation method and application of gold nanocage
  • Preparation method and application of gold nanocage

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preparation example Construction

[0025] The preparation method of gold nanocage is:

[0026] Dissolving CF3COOAg in ethylene glycol to prepare CF3COOAg mother liquor; weighing polyvinylpyrrolidone and dissolving it in ethylene glycol to prepare polyvinylpyrrolidone mother liquor;

[0027] Pipette concentrated hydrochloric acid and ethylene glycol to mix to prepare hydrochloric acid mother liquor; take NaHS and add it to ethylene glycol to prepare NaHS mother liquor,

[0028] Under the condition of 150°C-200°C, take NaHS mother liquor, ethylene glycol hydrochloride mother liquor, vinylpyrrolidone mother liquor, CF3COOAg mother liquor and react;

[0029] Transfer the reaction product obtained in the preceding steps to a centrifuge tube, add an acetone solution to wash and purify, then disperse the product in water, centrifuge to remove the supernatant, and wash to obtain a silver nanocubic solution;

[0030] Gold nanocages were prepared by electrochemical displacement method.

[0031] The application method i...

Embodiment 1

[0034] 1. Preparation of silver nanocubes.

[0035] A. will CF 3 COOAg was dissolved in ethylene glycol and formulated as CF at a concentration of 100 mg / mL 3 COOAg mother liquor: Weigh 300mg polyvinylpyrrolidone PVP (Mw=55kDa) and dissolve it in 15mL ethylene glycol to prepare 20mg / mL PVP mother liquor;

[0036] B. Pipette 5 μL of 37% concentrated hydrochloric acid and add it to 20 mL of ethylene glycol to prepare a 2.5 mmol / L mother liquor of hydrochloric acid; weigh NaHS (8.4 mg, 0.15 mmol) and add it to ethylene glycol to prepare a concentration of 10 mg / L mL of NaHS stock solution;

[0037] C. Take at 150°C, mix 0.6mL of NaHS mother liquor, after 2min, react with 5.0mL of ethylene glycol hydrochloride mother liquor, 12.5mL of PVP mother liquor, and 4.0mL of CF3COOAg mother liquor;

[0038] D. Transfer the reaction product obtained in step C to a centrifuge tube, add 2 to 3 times the volume of acetone solution to wash and purify, then add ultrapure water to ultrasonical...

Embodiment 2

[0050] 1. Preparation of silver nanocubes.

[0051] A. will CF 3 COOAg was dissolved in ethylene glycol and formulated as CF at a concentration of 10 mg / mL 3 COOAg mother liquor: Weigh 300mg polyvinylpyrrolidone PVP (Mw=55kDa) and dissolve it in 15mL ethylene glycol to prepare 20mg / mL PVP mother liquor;

[0052] B. Pipette 5 μL of 37% concentrated hydrochloric acid and add it to 20 mL of ethylene glycol to prepare a 2.5 mmol / L mother liquor of hydrochloric acid; weigh NaHS (8.4 mg, 0.15 mmol) and add it to ethylene glycol to prepare a concentration of 1 mg / L mL of NaHS stock solution;

[0053] C. Under the condition of 150°C, mix 0.6mL of NaHS mother liquor, after 2min, 5.0mL of ethylene glycol hydrochloride mother liquor, 12.5mL of PVP mother liquor, CF 3 COOAg mother liquor 4.0mL reaction;

[0054] D. Transfer the reaction product obtained in step C to a centrifuge tube, add 2 to 3 times the volume of acetone solution to wash and purify, then add ultrapure water to ultra...

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Abstract

The invention belongs to the technical field of CAR-T living body CT imaging, and particularly discloses a preparation method and application of a gold nanocage. The preparation method of the gold nanocage comprises the following steps of preparing CF3COOAg mother liquor; preparing polyvinylpyrrolidone mother liquor; preparing hydrochloric acid mother liquor; preparing NaHS mother liquor, and taking the NaHS mother liquor, ethylene glycol hydrochloride mother liquor, vinylpyrrolidone mother liquor and CF3COOAg mother liquor for reacting; transferring a reaction product obtained in the previousstep into a centrifugal tube, adding an acetone solution for washing and purifying, adding water into the product for dispersing, centrifuging to remove supernate, and washing to obtain a silver nanocubic solution; and preparing the gold nanocage by adopting an electrochemical replacement method. The gold nanocage is applied to a CAR-T living CT imaging agent. The method has the following beneficial effects that a cytokine storm is not easily caused; and CAR-T can be transfected at a time through electroporation, the operation process is simple, and the CAR-T has the advantages of high contrast and high specificity through CT imaging.

Description

technical field [0001] The invention belongs to the technical field of CAR-T in vivo CT imaging, and in particular relates to a preparation and application method of a gold nanocage for imaging. Background technique [0002] CAR-T cell therapy is to enable T cells to express certain co-stimulatory molecules and chimeric receptors targeting specific tumor antigens through genetic engineering technology, so as to have the ability to further activate and precisely kill specific tumor cells. If the amount of CAR-T is too large, it will cause a cytokine storm, and if it is not used enough, it will have no effect on the treatment of leukemia. Therefore, it is necessary to take effective measures to monitor the in vivo behavior of CAR-T. [0003] At present, the commonly used CAR-T tracking method is reporter gene transfection. Patent 201710648782.3 discloses a method for in vivo tracking and manual tracking of CAR-t labeling and application. This method requires the construction...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B22F9/24B22F9/16B22F1/00A61K49/04B82Y5/00B82Y30/00B82Y40/00
CPCB22F9/24B22F9/16A61K49/04B82Y5/00B82Y30/00B82Y40/00B22F1/0553B22F1/07B22F1/054
Inventor 唐宇翔梅恒胡豫陈昭昭姚惟琪
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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