Monascus liquid fermentation clear juice beverage
A technology of liquid fermentation and Monascus bacteria, applied in the direction of fungi, microorganisms, biochemical equipment and methods, etc., can solve problems affecting the sensory quality of beverages, easily turbidity or precipitation of beverage products, and reduce beverage stability, etc., to achieve product quality Clear, good stability, high sensory quality effect
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Embodiment 1
[0035] (1) Activation of strains: Insert Monascus purple ZSH-G-002 into the slant seed medium (glucose 5%, peptone 2%, soluble starch 2%, agar 2%), in a constant temperature incubator at 30°C Cultured in medium for 7 days.
[0036] (2) Preparation of fermentation medium: add 1% rice flour, 3% soybean flour, 0.1% 0.1% MgSO to clear water 4 ·7H 2 O, 0.1% KH 2 PO 4 , adjust the pH to 5 with lactic acid, and sterilize at 120°C for 30 minutes.
[0037] (3) Inoculate Monascus species, and carry out liquid fermentation step-by-step scale-up production: Inoculate the sterilized culture medium with Monascus purple ZSH-G-002, cultivate at 30°C for 24h-48h, and then at 24°C Ferment to 200h, obtain Monascus fermented liquid. Then detect the smell of fermentation broth, citrinin and Monacolin K content. Finally, the fermentation liquid after fermentation was sterilized for 30 min at 120°C, and filtered with a 750b filter cloth to obtain the Monascus fermented clear liquid.
[0038](...
Embodiment 2
[0040] (1) Activation of strains: Insert Monascus purple ZSH-G-002 into the slant seed medium (glucose 6%, peptone 2%, soluble starch 3%, agar 3%), in a constant temperature incubator at 28°C cultured in China for 8 days.
[0041] (2) Preparation of fermentation medium: add 1.5% rice flour, 3.2% soybean flour, 0.1% 0.1% MgSO to clear water 4 ·7H 2 O, 0.2% KH 2 PO 4 , adjust the pH to 5.5 with lactic acid, and sterilize at 120°C for 30 minutes.
[0042] (3) Inoculate Monascus strains, and carry out liquid fermentation step-by-step scale-up production: inoculate the sterilized culture medium with Monascus purple ZSH-G-002, cultivate at 30°C for 24h-48h, and then at 24°C Ferment to 220h, obtain Monascus fermented liquid. Then detect the smell of fermentation broth, citrinin and Monacolin K content. Finally, the fermentation liquid after fermentation was sterilized for 30 min at 120°C, and filtered with a 750b filter cloth to obtain the Monascus fermented clear liquid.
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Embodiment 3
[0045] (1) Activation of strains: insert Monascus purple ZSH-G-002 into the slant seed culture medium (glucose 7%, peptone 2%, soluble starch 3%, agar 3%), in a constant temperature incubator at 33°C cultured for 6 days.
[0046] (2) Fermentation medium preparation: add 2% rice flour, 3.5% soybean flour, 0.2% MgSO to clear water 4 ·7H 2 O, 0.1% KH 2 PO 4 , adjust the pH to 5.2 with lactic acid, and sterilize at 120°C for 30 minutes.
[0047] (3) Inoculate Monascus species, and carry out liquid fermentation step-by-step scale-up production: Inoculate the sterilized culture medium with Monascus purple ZSH-G-002, cultivate at 30°C for 24h-48h, and then at 24°C Ferment to 240h, obtain Monascus fermented liquid. Then detect the smell of fermentation broth, citrinin and Monacolin K content. Finally, the fermented fermentation liquid was sterilized at 120° C. for 30 minutes, and filtered with a 750 b filter cloth to obtain a Monascus fermented supernatant.
[0048] (4) Product...
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