A method for improving the formation rate of cell pools of stably transfected cell lines
A cell line and cell technology, applied in the field of cell culture, can solve problems such as inconvenient operation
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[0045] Embodiment 1 A kind of cultivation method of stably transfected cell line
[0046] In this example:
[0047] EX-CELL® CD CHO Fusion was purchased from Sigma, the article number is 24365C;
[0048] ExcellCHO Cloning Medium was purchased from Sigma, the article number is C6366;
[0049] BalanCD CHO Growth A was purchased from Irvine, Cat. No. 91128.
[0050] The empty vector of the plasmid to be transfected is pcGS3 from Merck; the plasmid to be transfected expresses the Fc fusion protein.
[0051] (1) Cell preparation
[0052] The day before electroporation, the host cell CHOZN® CHO K1 cells (purchased from SAFC) were sampled and counted, and 0.8×10 6 The cells / mL cell density was inoculated in shake flasks.
[0053] (2) Electrostaining
[0054]I. After counting the host cells in the logarithmic growth phase in step (1), count them with 1×10 7 cells / transfection calculates the cell volume required for each electroporation reaction. Centrifuge the cell suspension ...
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