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Hepatocellular carcinoma marker and detection kit

A technology of hepatocellular carcinoma and detection kit, which is applied in the field of biomedicine, can solve the problems that the expression level of transcription or translation products is easily interfered by experimental operation, detection system error, and large amount of detection tissue, so as to avoid experimental operation error and systematic Effects of errors, short detection times, and small tissue requirements

Active Publication Date: 2021-01-08
FOURTH MILITARY MEDICAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

However, most of the current detection of molecular markers has shortcomings such as the need for a large amount of detection tissue, the slow detection cycle, the expression level of transcription or translation products is easily interfered by experimental operations, and the detection system has large errors, so it cannot be fully applied in clinical practice.

Method used

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  • Hepatocellular carcinoma marker and detection kit
  • Hepatocellular carcinoma marker and detection kit
  • Hepatocellular carcinoma marker and detection kit

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Experimental program
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Embodiment

[0051] We performed miRNA and mRNA on normal liver (NH) tissues and liver cancer (HCC) tissues of 7 patients with liver cancer (from: Tangdu Hospital, Air Force Military Medical University, signed informed consent and related contracts to ensure that the use process is legal and compliant) Sequencing to identify miR-144 / miR-451a clusters as candidates.

[0052] The specific implementation steps are as follows:

[0053] 1) Use surgery or biopsy to collect liver tissue samples from the individual to be tested, about 2mm×2mm×1mm in size;

[0054] 2) Use proteinase K-containing tissue lysate (about 500 μl) to lyse the retained tissue, and digest at 55°C for 6 hours;

[0055] 3) After carefully blowing and mixing the fully lysed tissue lysate, carry out high-temperature centrifugation at 12,000 g at 4°C for 10 minutes to fully precipitate tissue and cell debris;

[0056] 4) Carefully suck out the supernatant, add an equal volume of Tris-saturated phenol (about 500 μl), invert up ...

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Abstract

The invention provides a new hepatocellular carcinoma marker and a detection kit. Early diagnosis can be performed on liver cancer more efficiently and accurately based on the marker, the tissue demand is small, the detection time is short, the detection accuracy is high, the operation method is simple, and the cost is lower. More importantly, the interaction of liver cancer and liver cancer immune microenvironment is comprehensively considered, and good reference and evaluation indexes are provided for further treatment strategies of liver cancer patients, such as immunotherapy selection targeting innate immune cells or targeting T lymphocytes, while the liver cancer early diagnosis marker is provided. The hepatocellular carcinoma marker and the detection kit have high reference value forearly diagnosis and immunotherapy selection of liver cancer, and are suitable for popularization and application.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and relates to a hepatocellular carcinoma marker and a detection kit. Background technique [0002] Hepatocellular carcinoma (hepatocellular carcinoma, HCC) is the most common malignant tumor of the digestive system, with a poor prognosis and a rising mortality rate. Multiple risk factors have the potential to cause genetic or phenotypic changes and lead to HCC progression, including viruses, fibrosis, metabolic disorders, and alcohol, among others. The complexity of etiology increases the difficulty of fully understanding the molecular mechanisms of HCC. [0003] According to the expression of specific molecular markers, the auxiliary diagnosis of hepatocellular carcinoma not only greatly improves the diagnostic accuracy of liver cancer, shortens the diagnosis time of liver cancer, but also can carry out molecular grading of hepatocellular carcinoma through molecular diagnosis. Facilitate ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/113
CPCC12Q1/6886C12Q2600/154C12Q2600/178
Inventor 刘雷赵俊龙李惠晨
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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