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Rice plant carbon isotope field in-situ calibration method

A carbon isotope and plant technology, applied in the field of 13C isotope labeling and quantification of rice plants, can solve the problem of lack of process control

Pending Publication Date: 2021-01-05
SHANGHAI ACAD OF AGRI SCI
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  • Claims
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Problems solved by technology

[0006] In view of the shortcomings of the prior art described above, the purpose of the present invention is to provide a carbon isotope field calibration method for rice plants, which is used to solve the lack of stable carbon isotope that can control the process and can be accurately quantified in the prior art. 13 C calibration method problem

Method used

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  • Rice plant carbon isotope field in-situ calibration method
  • Rice plant carbon isotope field in-situ calibration method

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Embodiment 1

[0174] Users get as figure 1 A carbon isotope field calibration system for rice plants is shown as follows: figure 2 In the calibration process shown, 48 hours before the start of the calibration, the base is first fixed in the soil of the paddy field to be calibrated, that is, the support frame is fixed in the soil of the paddy field to be calibrated, so that the paddy field area where the rice plants to be calibrated is located is enclosed in the middle of the base.

[0175] Then 24 hours before the official calibration, the marking box is sleeved on the base and closed, that is, the lower end of the side wall of the marking box is sleeved in the fixing groove of the base, the rice plants to be calibrated are covered in the marking box, and field water is poured into the base In the fixed groove of the marking box, the liquid seal between the marking box and the base is prevented to prevent gas exchange inside and outside the marking box, which will affect the accuracy of t...

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Abstract

The invention provides a rice plant carbon isotope field in-situ calibration system, which comprises the following components: a carbon marker release device, which comprises a motor, a screw, a sliding member and an injector, wherein the motor is in transmission connection with the screw and is used for driving the screw to rotate, the sliding part is connected with the screw rod and the injectorand is used for sliding under the driving of the screw rod so as to extrude and release the carbon marker in the injector; a marking box, wherein the marking box is hollow, an opening is formed in the bottom of the marking box, and the marking box is communicated with the injector through a carbon marker release tube; and a motor controller connected with the motor through a first signal line andused for sending a signal for driving the screw to rotate to the motor. The invention further provides a rice plant carbon isotope field in-situ calibration method. According to the rice plant carbonisotope field in-situ calibration method, 13C in-situ calibration is conducted on rice plants under the field in-situ condition, the process is controllable, measurement is accurate, human interference is reduced, and the 13C marking cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of plant isotope labeling, and relates to a carbon isotope field calibration method for rice plants, in particular to a carbon isotope calibration method used in the field in situ 13 A method for the precise quantification of rice plants by isotopic labeling of C. Background technique [0002] Stable carbon isotopes ( 13 C) Tracing technology is an important technical means in the study of rice carbon fixation, distribution and transformation. 13 C isotope tracer technology can carry out experiment implementation and measurement safely and reliably. according to 13 There are currently three main methods in the world for the difference in C marking frequency: single pulse marking, repetitive pulse marking and continuous marking. Pulse labeling is a bolus injection 13 Compared with other labeling techniques, pulse labeling has many advantages, and is suitable for the research on the distribution and trans...

Claims

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Application Information

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IPC IPC(8): G01N33/00
CPCG01N33/0098
Inventor 周胜王从孙会峰张继宁张鲜鲜
Owner SHANGHAI ACAD OF AGRI SCI
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