Chromogenic solid culture medium of nocardia seriolae as well as preparation method and application

A technology of Nocardia spp. and solid medium, which is applied in the field of chromogenic solid medium and its preparation for Nocardia spp., can solve the problems of difficult to achieve expected results, difficult separation, and high cost of separation. Achieve the effects of convenient observation, simple preparation method and high purity of isolated bacteria

Active Publication Date: 2020-12-29
SHENZHEN INST OF GUANGDONG OCEAN UNIV +2
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the molecular weight of the quorum effect substance and its similarity, the separation is difficult and the separation cost is high
In addition, the composition of bacteria quorum effect substances is complex, and it is difficult to achieve the expected effect by artificially adding a single substance

Method used

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  • Chromogenic solid culture medium of nocardia seriolae as well as preparation method and application
  • Chromogenic solid culture medium of nocardia seriolae as well as preparation method and application
  • Chromogenic solid culture medium of nocardia seriolae as well as preparation method and application

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Experimental program
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Effect test

Embodiment 1

[0043] This embodiment provides a kind of chromogenic solid medium of Nocardia yellowtail, and its preparation method comprises the following steps:

[0044] S1. Brain Heart Infusion Broth (BHI) liquid medium (purchased from Beijing Suo Laibao Technology Co., Ltd.) was used to inoculate Nocardia japonica, and cultivated to logarithmic growth phase (OD=0.5-0.6), and then centrifuge, take the supernatant, and sterilize it through a bacterial filter (the pore size of the filter membrane is 0.22 μm), and collect the filtrate to obtain the culture supernatant of Nocardia amberi.

[0045] S2. Weighing 30g of brain heart extract broth medium powder (purchased from Beijing Soleibao Technology Co., Ltd.), 15g of agar powder, 0.5g of escin, 0.05g of ferric citrate, 0.005g of antibiotic composition, 900g of distilled water, 180 mL of the culture supernatant of the above-mentioned Nocardia japonica was set aside. Wherein, the antibiotic composition is obtained by mixing 0.005 g of cefoxi...

Embodiment 2

[0049] This embodiment provides a kind of chromogenic solid medium of Nocardia yellowtail, and its preparation method comprises the following steps:

[0050] S1. Brain Heart Infusion Broth (BHI) liquid medium (purchased from Beijing Suo Laibao Technology Co., Ltd.) was used to inoculate Nocardia japonica, and cultivated to logarithmic growth phase (OD=0.5-0.6), and then centrifuge, take the supernatant, and sterilize it through a bacterial filter (the pore size of the filter membrane is 0.22 μm), and collect the filtrate to obtain the culture supernatant of Nocardia amberi.

[0051] S2. Weighing 45g of brain-heart extract broth medium powder (purchased from Beijing Suo Laibao Technology Co., Ltd.), 25g of agar powder, 1.5g of escin, 0.5g of ferric citrate, 0.09g of antibiotic composition, 1100g of distilled water, 55 mL of the culture supernatant of the above-mentioned Nocardia japonica was set aside. Wherein, the antibiotic composition is obtained by mixing 0.02 g of cefoxit...

Embodiment 3

[0055] This embodiment provides a kind of chromogenic solid medium of Nocardia yellowtail, and its preparation method comprises the following steps:

[0056] S1. Brain Heart Infusion Broth (BHI) liquid medium (purchased from Beijing Suo Laibao Technology Co., Ltd.) was used to inoculate Nocardia japonica, and cultivated to logarithmic growth phase (OD=0.5-0.6), and then centrifuge, take the supernatant, and sterilize it through a bacterial filter (the pore size of the filter membrane is 0.22 μm), and collect the filtrate to obtain the culture supernatant of Nocardia amberi.

[0057] S2. Weighing 35g of brain-heart extract broth medium powder (purchased from Beijing Suo Laibao Technology Co., Ltd.), 18g of agar powder, 0.7g of escin, 0.4g of ferric citrate, 0.06g of antibiotic composition, 1000g of distilled water, 100 mL of the culture supernatant of the above-mentioned Nocardia japonica was set aside. Wherein, the antibiotic composition is obtained by mixing 0.005 g of cefox...

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Abstract

The invention is applicable to the field of fish pathogenic microbiology, and provides a chromogenic solid culture medium of nocardia seriolae as well as preparation method and application. The chromogenic solid culture medium comprises the following components: brain heart leachate broth culture medium powder, agar powder, esculin, ferric citrate, an antibiotic composition, distilled water and culture supernatant of nocardia seriolae. The culture medium with the characteristics of short separation time, high sensitivity, high isolated bacterium purity, simple preparation method and the like can be prepared by adding the esculin, the ferric citrate, the culture supernatant of the nocardia seriolae in the logarithmic phase and the antibiotic composition on the basis of the basic formula ofthe brain heart leachate broth agar culture medium. The culture medium has the characteristics of high separation efficiency, high separation purity, convenience in observation and the like when beingused for separating nocardia seriola, and can be effectively applied to the separation of nocardia seriola in clinical sample.

Description

technical field [0001] The invention belongs to the field of fish pathogenic microbiology, and in particular relates to a color-developing solid culture medium of Nocardia amberii and its preparation method and application. Background technique [0002] Yellowtail Nocardia is the main pathogen of nocardiosis in aquaculture, widely distributed in the ocean, soil, activated sludge, decaying vegetation and animal excrement. It can mainly cause white nodules in the internal organs of the fish, resulting in slow movement of the fish, loss of appetite, low immunity, and death. At present, the fishes reported in the literature to be infected by Nocardia spp. mainly include: largemouth bass, pompano ovata, snakehead and so on. Nocardia spp. is a facultative aerobic Gram-positive bacterium, and its content in water is not high. When aquatic animals are weak and have low immunity, Nocardia spp. can pass through gills or wounds Infect. The disease has a long period of onset, high in...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/365
CPCC12N1/20
Inventor 夏立群侯素莹鲁义善王文基陈国权
Owner SHENZHEN INST OF GUANGDONG OCEAN UNIV
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