Inoculation method and special apparatus for plant tissue cell culture

A plant tissue and cell culture technology, applied in the fields of botanical equipment and methods, plant regeneration, horticultural methods, etc., can solve the problems of extensive operation, high operation and environmental requirements, easy to contaminate bacteria, etc., to improve efficiency and reduce labor. Strength, the effect of simplifying the operation process

Inactive Publication Date: 2003-09-17
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The biggest problem in plant tissue culture is that it is easy to infect bacteria, so it has high requirements on operation and environment
Traditional immobilized enzyme and immobilized cell reactors are not suitable for plant tissue culture due to their extensive operation and high packing density

Method used

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  • Inoculation method and special apparatus for plant tissue cell culture
  • Inoculation method and special apparatus for plant tissue cell culture
  • Inoculation method and special apparatus for plant tissue cell culture

Examples

Experimental program
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Effect test

Embodiment 1

[0032] according to figure 1 Make one with 3 transfer heads 4, the inoculum 11 is filled with distilled water at the bottom of the reactor. A reactor whose external dimensions are 25cm in height and 20cm in diameter is used, and four layers of stainless steel wire mesh are used as trays and trays. The method of traction is: the rotating shaft is connected to the soft connecting piece 3 of the soft wire to rotate and pack up the soft wire with the rotating shaft, and the soft wire drives the steel wire mesh, which can be promoted layer by layer. The inoculum material 10 that uses has the cut section of vegetable stem, the cut section of orange straw peel and the cut section of potato aseptic tissue cultured seedling and sends into the reactor from inoculation port 6 and floats on the inoculum liquid surface. After the first layer of trays 2 were immersed in the inoculum 11, oxygen was introduced into the reactor through the gas distributor 5, and the inoculum 11 was distilled ...

Embodiment 2

[0034] according to figure 1 Make one with 8 layers of trays 2 as a supporting frame, and the soft connectors 3 between the trays 2 are connected by chains. In addition, there is a removable inoculation net, which can take out the reactor. Other structures are similar to Scheme 1. Before inoculation, each layer of supporting trays is stacked on the bottom of the reactor. Put the whole reactor in a sterile environment, or only insert the inoculation port into the aseptic operation room, open the inoculation port; send the inoculation net into the reactor under aseptic conditions, place it on the first support tray, and inoculate ; Promote the first layer of trays to make it higher than the inoculation port, while the second layer of supporting trays is still in a position suitable for placing movable trays; repeat the previous operation and continue to promote the first layer of trays to drive the second layer of trays layer tray, lift it up. Follow this sequence until all t...

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Abstract

The invention relates to a plant tissue cell culture inoculation method and a special device. The device adopts multi-layer trays in the reactor, and the plates are connected by soft joints. The inoculation port is located at the lower part of the side wall of the reactor; there is one or several medium delivery ports on the top of the reactor, and a gas distributor at the bottom; The external conveying device communicates with the conveying port and the culture medium, and plays the role of conveying the culture medium during the cultivation and reaction process. When inoculating, lift the tray layer by layer under aseptic conditions, and inoculate layer by layer; the invention is suitable for plant tissue cell culture, immobilized enzyme, immobilized cell reaction and other biochemical processes. The fixed-bed reactor is multi-layered to make full use of space; the inoculation operation is greatly simplified, the inoculation is uniform, and aseptic is achieved.

Description

technical field [0001] The invention relates to an inoculation method and a special device for plant tissue cell culture. Background technique [0002] Fixed bed bioreactor was originally applied to the bioreaction device for enzymatic reaction using immobilized enzyme or immobilized cells as biocatalyst. In recent years, with the development of plant tissue culture technology, the design of new culture reactors has become more and more important. According to the experience of plant tissue culture, in many cases, the immobilized culture method is beneficial to the growth and development of plant materials. Therefore, fixed-bed reactors are increasingly used as plant tissue culture devices. [0003] The traditional method of plant tissue culture is: in shake flasks or small containers, submerge the culture material with a shallow liquid medium or place the material on a solid medium for fixed culture. The cumbersome operation and low efficiency of this method are not cond...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 郝震龙欧阳藩陈正华刘志岩王玉春陈受宜
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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