A temperature measurement method based on rare earth luminescence lifetime temperature probe

Abstract

The invention discloses a temperature measurement method of an internal quenching type rare earth luminescence lifetime temperature probe based on the change of fluorescence lifetime to realize temperature detection. The center of the present invention is doped with rare earth trivalent ions Yb 3+ and B 3+ The nano-core, surrounded by an inert shell, where Yb 3+ and B 3+ There is a phonon-assisted energy transfer process between rare earth ions. Because phonons participate in the energy transfer process, the lifetime of rare earth ions changes with temperature changes. The inert shell can protect the lifetime of the luminescent core from changing with the environment (except temperature) And the role of the change occurs, the luminescence band is in the 700-1700nm region, and the water solubility can be improved by modifying the ligand on the surface. The luminescence lifetime temperature probe of the present invention is a near-infrared luminescence lifetime temperature probe that can accurately measure temperature in different complex environments (including living bodies), and has internal quenching temperature sensitivity, near-infrared band luminescence, nanometer size level, accurate and quantifiable, Good stability and dispersion.

Description

technical field [0001] The invention relates to the field of luminescent nanometer probes and their applications, in particular to a temperature measurement method based on a rare earth luminescence lifetime temperature probe. Background technique [0002] Life activities in nature are always accompanied by changes in temperature, and during the evolution of organisms, the tolerance of different organisms to temperature is also quite different. For example, psychrophilic bacteria can survive at minus 15°C, while thermophilic bacteria can survive at temperatures as high as 110°C. For mammalian warm-blooded animals, metabolism and nerve signal transmission are always accompanied by temperature changes, while cells are a tiny unit that reflects temperature regulation, and the functions of many cells reflect the macroscopic temperature of living bodies. Finally, it can resist temperature changes within a certain range externally, and maintain a normal body temperature within a ...

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Problems solved by technology

However, because the fluorescence signals of different bands are absorbed and scattered by different tissues, in different complex environments, the degree of attenuation of the fluorescence signals of different bands of the specific temperature probe is also different, thus affecting the intensity of the fluorescence signal. accurate readout of

[0004] At present, the research and development of luminescent probes based on the luminescence lifetime as a temperature detection signal is very little. Some researchers use nano-gold clusters as temperature probes. The fluorescence lifetime of gold clusters is greatly affected by the external environment other than temperature, and cannot accurately respond. The temperature of the environment and its lifetime are at the nanosecond level, so the requirements for both the excitation light and the detector are relatively high

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