Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Dendrobium officinale seedling raising method

A technology of Dendrobium officinale and explants, applied in the field of bioengineering, can solve problems such as poor quality of Dendrobium officinale, achieve the effects of reducing production cost, improving reproduction speed and survival rate, and solving poor quality

Inactive Publication Date: 2020-12-18
灵山县山霖生物技术有限公司
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved in the present invention is to overcome the defects of the prior art, provide a seedling raising method of Dendrobium candidum, which can improve the reproduction speed and survival rate of Dendrobium candidum, solve the technical problem of poor quality in the current Dendrobium candidum seedling cultivation process, greatly improve the quality of Dendrobium candidum. Reduce production costs and meet the needs of large-scale, large-scale seedling cultivation

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Dendrobium officinale seedling raising method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The invention provides a kind of seedling raising method of Dendrobium officinale, comprising the following steps:

[0019] A: Collect robust wild Dendrobium officinale in the wild, remove the leaves, use the stems as explants, wash them with clean water, soak them in 75% alcohol solution for 2-3 minutes, and rinse them with sterile water for 3 minutes -5min, put the explants into a sterilized bottle, soak in 0.5% sodium hypochlorite for 4-7min, rinse with sterile water for 5-9min, and then inoculate in the following sterile induction medium: MS+ NAA+apple puree 50g / L+mashed potato 30g / L+banana juice 20g / L+sucrose 30g / L+kinetin 1mg / L, at a temperature of 24-29°C, light time of 8-14h, intensity of 1800-2400x, culture 80- A large number of protocorms can be germinated after 100 days;

[0020] B: using the same sterile induction medium to transfer a large number of protocorms that have germinated, once every 60-80 days, for a total of 2 times, to obtain fruit pods of Dend...

Embodiment 2

[0027] The invention provides a kind of seedling raising method of Dendrobium officinale, comprising the following steps:

[0028] A: Collect robust wild Dendrobium officinale in the wild, remove the leaves, use the stems as explants, wash them with clean water, soak them in 75% alcohol solution for 2-3 minutes, and rinse them with sterile water for 3 minutes -5min, put the explants into a sterilized bottle, soak in 0.4% sodium hypochlorite for 4-7min, rinse with sterile water for 5-9min, and then inoculate in the following sterile induction medium: MS+ NAA+apple puree 20g / L+mashed potato 50g / L+banana juice 50g / L+sucrose 30g / L+kinetin 4mg / L, at a temperature of 24-29°C, light time of 8-14h, intensity of 1800-2400x, culture 80- A large number of protocorms can be germinated after 100 days;

[0029] B: using the same sterile induction medium to transfer a large number of protocorms that have germinated, once every 60-80 days, for a total of 2 times, to obtain fruit pods of Dend...

Embodiment 3

[0036] The invention provides a kind of seedling raising method of Dendrobium officinale, comprising the following steps:

[0037] A: Collect robust wild Dendrobium officinale in the wild, remove the leaves, use the stems as explants, wash them with clean water, soak them in 75% alcohol solution for 2-3 minutes, and rinse them with sterile water for 3 minutes -5min, put the explants into a sterilized bottle, soak in 0.1% sodium hypochlorite for 4-7min, rinse with sterile water for 5-9min, and then inoculate in the following sterile induction medium: MS+ NAA+ apple puree 20g / L+ mashed potato 20g / L+ banana juice 50g / L+ sucrose 30g / L+ kinetin 4mg / L, at a temperature of 24-29°C, a light time of 8-14h, an intensity of 1800-2400x, culture 80- A large number of protocorms can be germinated after 100 days;

[0038] B: using the same sterile induction medium to transfer a large number of protocorms that have germinated, once every 60-80 days, for a total of 2 times, to obtain fruit po...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a dendrobium officinale seedling raising method. The method comprises the following steps of collecting wild robust dendrobium officinale, removing leaves in the wild robust dendrobium officinale, taking stems as explants, disinfecting, inoculating into a sterile induction culture medium, and culturing for 80-100 days to germinate a large number of protocorms; transferringa large number of germinated protocorms by adopting the same sterile induction culture medium, transferring once every 60-80 days, and transferring twice in total to obtain pods of dendrobium officinale; transplanting pods of the dendrobium officinale into a culture medium, fixedly spraying a nutrient solution for three times every day, culturing seedlings for 38-42 days, and performing acclimatization and transplantation; and transplanting the culture seedlings into a greenhouse for cultivation, spraying a sterilization nutrient solution every other day, and keeping the temperature of the greenhouse at 21-30 DEG C. According to the method, the propagation speed and the survival rate of the dendrobium officinale can be increased, the technical problem that the quality is poor in the current dendrobium officinale seedling raising process is solved, the production cost is greatly reduced, and the requirement for large-area and large-scale seedling raising is met.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a method for growing seedlings of dendrobium officinale. Background technique [0002] Dendrobium candidum is a herbaceous plant of the genus Dendrobium in the family Orchidaceae. At present, the wild Dendrobium candidum has small seeds, slow growth, and a large amount of artificial mining, so that the output of wild Dendrobium candidum is very small. Therefore, the current Dendrobium candidum is artificially cultivated. Cultivate, but the survival rate of the dendrobium candidum that artificial culture handles is not high at present, and the rhizome growing way is not strong, causes the cultivation cost to improve, has had a strong impact on the quality of dendrobium candidum seedling cultivation. Contents of the invention [0003] The technical problem to be solved in the present invention is to overcome the defects of the prior art, provide a seedling raising method o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00A01G24/10A01G24/15A01G24/22A01G24/23A01G24/28A01H4/00
CPCA01G31/00A01H4/001A01H4/008A01G24/10A01G24/15A01G24/22A01G24/23A01G24/28
Inventor 林雨覃步容曹晓明
Owner 灵山县山霖生物技术有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com