Thyroid cancer detection products and applications based on high-throughput sequencing

Abstract

The invention belongs to the field of biotechnology, and specifically relates to a combination of primers for thyroid cancer detection, including DNA PCR primers and RNA PCR primers. The DNA PCR primers include ATM gene mutation detection primer pairs, BRAF gene mutation detection primer pairs, and HRAS gene mutation detection primers. Detection primer pair, NRAS gene mutation detection primer pair, PTEN gene mutation detection primer pair, RET gene mutation detection primer pair, TERT gene mutation detection primer pair, TG gene mutation detection primer pair, TP53 gene mutation detection primer pair, TSHR gene mutation detection Primer pair, TTN gene mutation detection primer pair; said RNA PCR primers include RET gene fusion detection primer pair, NTRK3 gene fusion detection primer pair, BRAF gene fusion detection primer pair. The invention can simultaneously detect DNA mutation and RNA fusion. The amount of sequencing data for a single sample detection is reduced to less than 0.2M reads, which greatly reduces the cost of sequencing. Since DNA mutation and RNA fusion are tested in the same system, the workload of operation is reduced.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a thyroid cancer detection product and application based on a high-throughput sequencing method. Background technique [0002] Thyroid cancer is the most common malignant tumor in the endocrine system and head and neck tumors. Thyroid cancer is currently the fastest-rising malignant tumor. According to tumor origin and differentiation, thyroid cancer is further divided into papillary thyroid carcinoma (PTC), follicular thyroid carcinoma (FTC), medullary thyroid carcinoma (MTC) and thyroid carcinoma. Undifferentiated carcinoma (anaplastic thyroid carcinoma, ATC). Among them, PTC and FTC are collectively called differentiated thyroid cancer (Differentiated thyroid cancer, DTC). Different pathological types are obviously different in their pathogenesis, biological behavior, tissue morphology, clinical manifestations, treatment methods, and prognosis. [0003] During the tr...

AI Technical Summary

Problems solved by technology

[0012] Disadvantages: single-point detection, incomplete coverage of thyroid gene detection sites, increased workload of combined detection, high cost of reagents

[0019] Disadvantages: A large amount of data is generated. Although the cost of whole gene sequencing is relatively low, the overall test cost is still high, the operation is complicated, and the test cycle is long. It usually takes 4-5 days to get the results.

ARMS-PCR technology can only detect DNA mutations at a single point, and the types of detection are not complete, so there are limitations. In addition, the thyroid fine needle biopsy has fewer materials, and the total amount of purified nucleic acid is limited. Usually, DNA detection can only be performed alone. Point detection cannot meet more DNA mutation point detection and RNA fusion detection at the same time, and cannot meet the needs of clinical detection

Hybrid capture sequencing technology enables high-throughput sequencing technology to meet DNA mutation and gene fusion, but due to the large amount of sequencing data required, the cost is still high, the operation is complicated, and the experimental period is long

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