A kind of method of bacillus pasturella dsm33 strengthens regenerated fine aggregate

A technology of Bacillus pasteurii and regenerated fine aggregates, applied in the intersection of microbiology and solid waste resource utilization, in the field of building materials, it can solve the problem of increasing the potential cost of the modification process, limiting the scope of application of the modification process, and regenerating fine aggregates. Less aggregate modification and other problems, to achieve the effect of improving modification efficiency and effect, reducing operating cost, and good stress resistance

Active Publication Date: 2022-04-22
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most of the existing modification processes are aimed at regenerated coarse aggregate, and there is less modification of regenerated fine aggregate.
At the same time, the existing process often needs to cooperate with vibration or add other auxiliary chemical substances to improve the effect, which greatly limits the scope of application of the modification process and increases the potential cost of the modification process
In addition, due to the immaturity of the existing process research, the length of some processes is too long, the modification efficiency is low, and it is not suitable for practical application

Method used

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  • A kind of method of bacillus pasturella dsm33 strengthens regenerated fine aggregate
  • A kind of method of bacillus pasturella dsm33 strengthens regenerated fine aggregate
  • A kind of method of bacillus pasturella dsm33 strengthens regenerated fine aggregate

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Embodiment 1

[0030] This embodiment includes the following steps:

[0031] (1) Weigh 20 g of tryptone, 5 g of sodium chloride, 20 g of urea, and 1000 mL of deionized water to prepare a proliferation medium, and adjust the pH value to 9.5 by adding NaOH solution and HCl solution dropwise. After autoclaving at 121° C. for 20 minutes, Bacillus pasteurianus DSM33 was inoculated, shaken at 30° C. for 24 hours, and the shaking frequency was 130 rpm.

[0032] (2) Pass the regenerated fine aggregate through a 0.075mm sieve and wash it with water. The particle size ranges from 0.075mm to 4.75mm. Weigh 1000g and add it to 1L of the above-mentioned bacterial proliferation culture solution, and culture it statically at 30°C for 24h.

[0033] (3) Weigh 3 g of tryptone, 2.12 g of sodium carbonate, 5 g of ammonium chloride, 20 g of urea, 0.5 mol of calcium acetate, and 1000 mL of deionized water to prepare a precipitation medium. The above-mentioned regenerated fine aggregate was taken out from the bact...

Embodiment 2

[0040] This embodiment includes the following steps:

[0041] (1) Take 20 g / L tryptone, 5 g / L sodium chloride, and 20 g / L urea as standard to weigh and configure the proliferation medium, and adjust the pH value to 9.5 by adding NaOH solution and HCl solution dropwise. After autoclaving at 121° C. for 20 minutes, Bacillus pasteurianus DSM33 was inoculated, shaken at 30° C. for 24 hours, and the shaking frequency was 130 rpm.

[0042] (2) Prepare construction waste into recycled fine aggregate and wash it with water. The sieved particle size ranges from 0.6mm to 4.75mm. The ratio of aggregate to proliferation medium is 100g:100mL, and the aggregate is put into bacterial proliferation culture medium cultured at 30°C for 24 hours.

[0043] (3) Take tryptone 3g / L, sodium carbonate 2.12g / L, ammonium chloride 5g / L, urea 20g / L, calcium acetate 0.5mol / L as the standard and prepare the precipitation medium. The above-mentioned regenerated fine aggregate is taken out from the bacterial ...

Embodiment 3

[0051] This embodiment includes the following steps:

[0052] (1) Take 20 g / L tryptone, 5 g / L sodium chloride, and 20 g / L urea as standard to weigh and configure the proliferation medium, and adjust the pH value to 9.5 by adding NaOH solution and HCl solution dropwise. After autoclaving at 121° C. for 20 minutes, Bacillus pasteurianus DSM33 was inoculated, shaken at 30° C. for 24 hours, and the shaking frequency was 130 rpm.

[0053] (2) The construction waste is prepared into recycled fine aggregate and washed with water, and the sieved particle size ranges from 0.075 mm to 4.75 mm, and is equally divided into two parts for later use. And take tryptone 3g / L, sodium carbonate 2.12g / L, ammonium chloride 5g / L, urea 20g / L, calcium acetate 0.5mol / L as the standard, weigh and configure the precipitation medium, set aside.

[0054] (3) The first aggregate is recorded as one-time modified aggregate, and the ratio of aggregate and proliferation medium is 100g:100mL. Put the aggregate...

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Abstract

The invention provides a method for strengthening regenerated fine aggregate by using Bacillus pasteurianus DSM33, comprising the following steps of configuring a proliferation medium, adjusting the pH to 9.0-9.5 by adding NaOH solution and HCl solution dropwise, sterilizing and cooling to Inoculate Bacillus pasteurianum after room temperature, shake and cultivate at 28-32°C and 130rpm to obtain a bacterial culture solution that has completed the proliferation; add dry regenerated fine aggregate to the bacterial culture solution that has completed the proliferation, and place it at 28-32°C. Cultivate at 32°C; take out the soaked regenerated fine aggregate and put it into the precipitation medium, and let it stand at 28-32°C for cultivation; after completing the above operations, dry the regenerated fine aggregate to obtain a strengthened Regenerated fine aggregate; repeated strengthening can be carried out on the once strengthened aggregate. The regenerated fine aggregate prepared by the method of the invention increases in weight and significantly reduces water absorption. The compressive strength and flexural strength of the regenerated mortar prepared from the strengthened regenerated fine aggregate are significantly improved, and the shrinkage performance and carbonization resistance are remarkably improved.

Description

technical field [0001] The invention relates to the interdisciplinary technical field of building materials, microbiology and solid waste resource utilization, in particular to a method for strengthening regenerated fine aggregate with Bacillus pasteurianum DSM33. Background technique [0002] my country is currently in the process of vigorous development of the construction industry and the construction of a large number of urban infrastructure. As the most important building material, the average annual output of commercial concrete has exceeded 2.5×10 9 m 3 , which means the rapid consumption of natural sand and gravel resources, and about 32 billion to 50 billion tons of sand and gravel resources in the world are used for industrial production every year. At the same time, it cannot be ignored that the annual construction waste generated in my country has exceeded 3 billion tons, and the utilization rate of resources is less than 10%. The shortage of resources and the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C04B20/02
CPCC04B20/023C04B18/16
Inventor 赵羽习冯璋曜曾维来
Owner ZHEJIANG UNIV
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