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Polygonatum cyrtonema rhizome tissue culture seedling sterilization, insect prevention and rooting powder and preparation method thereof

A technology of rooting powder and rhizomes, applied in botany equipment and methods, biocides, fungicides, etc., can solve the problems of easy browning, difficulty, and difficult disinfection, and achieve high seedling growth rate and ensure seedling growth , the effect of high survival rate

Inactive Publication Date: 2020-12-08
阳山县三连阳生态农林开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But no one has successfully carried out it at present, mainly because the morphological structure of Polygonatum multiflora plants is special, the rhizome flesh grows in the soil, the explants are difficult to sterilize and become sterile propagation materials, and the rhizomes contain Rich in polysaccharides and other metabolic secondary substances, it is easy to brown, and Polygonatum polyflora has the biological characteristics of dormancy. It is very difficult to adopt the bud proliferation method like most plant tissue cultures to continuously expand the number of buds.

Method used

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  • Polygonatum cyrtonema rhizome tissue culture seedling sterilization, insect prevention and rooting powder and preparation method thereof
  • Polygonatum cyrtonema rhizome tissue culture seedling sterilization, insect prevention and rooting powder and preparation method thereof
  • Polygonatum cyrtonema rhizome tissue culture seedling sterilization, insect prevention and rooting powder and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The present embodiment provides a plant tissue culture sterilization abt pest, the formulation composition shown in Table 1 abt.

[0039] Table 1 special formula and sterilize insects abt description table

[0040]

[0041] The above-described pest control plant tissue culture sterilization abt preparation process includes the following steps:

[0042] S1: Weigh the appropriate starting material:

[0043]1. Weigh Talc: wear masks and disposable plastic gloves, said electronic weighing 990 g talc, placed 40cm × 60cm stainless steel surgical tray.

[0044] 2.90% O, O- dimethyl - (2,2,2-trichloro-1-hydroxyethyl) phosphonate weighed: weighed 90% O, O- dimethyl - (2,2,2 - trichloro-1-hydroxyethyl) phosphonate 5g, placed in a plastic 1L beaker, added after 100mL of purified water were dissolved, poured uniformly on said take good talc, and stirred constantly with a glass rod.

[0045] 3. Nano silver (Ag + ) D weighed stock solution: Take 40mL graduated cylinder with nano silver...

Embodiment 2

[0051] The present embodiment provides a plant tissue culture sterilization abt pest, the formulation of ABT material component comprising: 400ppm IBA, 3ppm 2.4-D, 20ppm 6BA, 900ppm KH 2 PO 4 , 900ppm KNO 3 , 50ppm FeSO 4 · 7h 2 0,40ppm H 3 BO 3 , 30mL 100ppm of Ag + Solution, 4500ppm O, O- dimethyl - (2,2,2-trichloro-1-hydroxyethyl) phosphonate and adhesion agent.

[0052] The above-described pest control plant tissue culture sterilization abt preparation process includes the following steps:

[0053] S1: The corresponding starting weighed proportion:

[0054] 1. Weigh Talc: wear masks and disposable plastic gloves, said electronic weighing 990 g talc, placed 40cm × 60cm stainless steel surgical tray;

[0055] 2.90% O, O- dimethyl - (2,2,2-trichloro-1-hydroxyethyl) phosphonate weighed: weighed 90% O, O- dimethyl - (2,2,2 - after-trichloro-1-hydroxyethyl) phosphonate 4.5g, placed in a plastic 1L beaker, 100mL of purified water was added to dissolve uniformly poured into the weighe...

Embodiment 3

[0062] The present embodiment provides a plant tissue culture sterilization abt pest, the formulation of ABT material component comprising: 600ppm IBA, 5ppm 2.4-D, 40ppm 6BA, 1100ppm KH 2 PO 4 , 1100ppm KNO 3 , 150ppm FeSO 4 · 7h 2 0,60ppm H 3 BO 3 , 50mL 100ppm of Ag + Solution, 5500ppm O, O- dimethyl - (2,2,2-trichloro-1-hydroxyethyl) phosphonate and adhesion agent.

[0063] The above-described pest control plant tissue culture sterilization abt preparation process includes the following steps:

[0064] S1: Weigh the appropriate starting material:

[0065] 1. Weigh Talc: wear masks and disposable plastic gloves, said electronic weighing 990 g talc, placed 40cm × 60cm stainless steel surgical tray.

[0066] 2.90% O, O- dimethyl - (2,2,2-trichloro-1-hydroxyethyl) phosphonate weighed: weighed 90% O, O- dimethyl - (2,2,2 - trichloro-1-hydroxyethyl) phosphonate 5.5g, placed in a plastic 1L beaker, added after 100mL of purified water were dissolved, poured uniformly on said take good ...

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PUM

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Abstract

The invention discloses special sterilization insect-prevention rooting powder for polygonatum cyrtonema tissue culture industrialized rhizome propagation and seedling raising. The rooting powder is prepared from IBA, 2.4-D, 36BA, KH2PO4, KNO3, FeSO4. 7H20, H3BO3, a nano-silver solution, O, O-dimethyl-(2, 2, 2-trichloro-1-hydroxyethyl)phosphonate crystals and 99% talcum powder. The rooting powderdisclosed by the invention is used for transplanting polygonatum cyrtonema test-tube plantlets subjected to tissue culture and industrialized seedling production, accelerates rooting of tubers and germination and growth of buds, plays a role in efficient sterilization, ensures rooting and seedling formation of the rhizomes, improves the transplanting survival rate of the test-tube plantlets of therhizomes, and improves the yield of polygonatum cyrtonema. And the rooting powder can be adopted in other plants propagated by rhizomes.

Description

Technical field [0001] The present invention relates to the technical field cultivation, in particular to a rhizomes of Polygonatum plant tissue culture sterilization pest abt. Background technique [0002] Rhizome plants (such as: multi-cyrtonema Polygonatumcyrtonema Hua, potato Solanumtuberosum L., ginger Zingiber officinale, little turmeric DioscoreaZingiberensisC.H.Wright, yam Dioscoreaoppositifolia L.) common flowering species without knot or knot species of small, difficult to germinate . It is difficult to adopt the seeds for large-scale seed production. Currently, in addition to potatoes, the vast majority of other species, can not adopt a large number of plant tissue culture propagation techniques seedlings. Rhizome meristem propagation is the use of more propagation method, but this method of propagation of the need to consume a large amount of original material roots, and this method of cultivation of seedlings susceptible to degradation, and because of a viral infecti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/38A01N39/04A01N43/90A01N59/26A01N59/00A01N59/14A01N59/16A01N57/20A01P21/00A01P1/00A01P3/00A01P7/04A01G7/06A01G13/00A01G9/029
CPCA01G7/06A01G13/00A01N39/04A01N43/38A01N43/90A01N57/20A01N59/00A01N59/14A01N59/16A01N59/26A01G9/0299
Inventor 张华通张炜林云斌黄剑雄
Owner 阳山县三连阳生态农林开发有限公司
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