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Neural stem cell exosome and application thereof in treating neurodegenerative diseases

A neural stem cell, neurodegenerative technology, applied in nervous system cells, nervous system diseases, animal cells, etc., can solve the problem of exosome application without neural stem cells

Inactive Publication Date: 2020-12-01
张文化
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There are currently no therapeutic applications of neural stem cell exosomes in the treatment of neurodegenerative diseases

Method used

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  • Neural stem cell exosome and application thereof in treating neurodegenerative diseases
  • Neural stem cell exosome and application thereof in treating neurodegenerative diseases
  • Neural stem cell exosome and application thereof in treating neurodegenerative diseases

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Experimental program
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Embodiment Construction

[0020] The substantive content of the present invention will be described in detail below in conjunction with the embodiments, but the protection scope of the present invention is not limited thereto.

[0021] 1. Experimental materials

[0022] Human neural stem cells (hNSCs) were purchased from Shenzhen Haodi Huatuo Biotechnology Co., Ltd., and are currently in the third generation.

[0023] H-DMEM medium was purchased from Thermo Fisher Scientific (China) Co., Ltd.

[0024] RPMI 1640 medium and fetal bovine serum were purchased from Gibco.

[0025] ExoQuick kit was purchased from Beijing Bomeisi Biotechnology Co., Ltd.

[0026] BCA kit was purchased from Biyuntian.

[0027] The purity of Compound C is ≥98%, and its chemical structure is as follows:

[0028]

[0029] 2. Experimental method

[0030] 1. Recovery, culture and passage of hNSCs

[0031] Take out the hNSCs cryopreservation tube, immerse the tube body in a 37°C water bath, shake gently, dissolve more than 9...

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Abstract

The invention discloses a neural stem cell exosome and an application thereof in treating neurodegenerative diseases. The invention finds that the neural stem cell exosome can improve the activity ofnerve cells by inhibiting apoptosis caused by A beta 25-35 on the nerve cells PC12; and the Compond C-loaded neural stem cell exosome can also improve the activity of nerve cells by inhibiting apoptosis caused by A beta 25-35 on nerve cells PC12, and the anti-apoptosis activity is obviously superior to that of unloaded neural stem cells. Therefore, the neural stem cell exosome and the Compond C-loaded neural stem cell exosome can be prepared into a medicine for treating the Alzheimer's disease.

Description

technical field [0001] The invention belongs to the biological field and relates to the application of stem cells in the treatment of diseases, in particular to a neural stem cell exosome and its application in the treatment of neurodegenerative diseases. Background technique [0002] Alzheimer's disease (AD) is a neurodegenerative disease characterized by progressive memory decline, cognitive behavioral impairment and other neuropsychiatric symptoms. Neuronal loss, senile plaques, neurofibrillary tangles. [0003] Current studies suggest that Aβ deposition is the common path of various factors leading to AD. Hydrolysis of β-amyloid precursor produces Aβ. Under normal circumstances, the generation and hydrolysis of Aβ are in a dynamic balance, and only a very small amount is expressed in the brain. When the following situations occur, such as mutation or overexpression or degeneration of the APP gene, the reactivity to nerve growth factors in the body decreases, and the ou...

Claims

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Application Information

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IPC IPC(8): C12N5/0797A61K35/30A61K31/573A61P25/28A61P25/00
CPCA61K31/573A61K35/30A61P25/00A61P25/28C12N5/0623A61K2300/00
Inventor 不公告发明人
Owner 张文化
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