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Application of TGA7 gene in regulation and control of flowering period of plant

A gene and plant technology, applied in the application field of TGA7 gene in regulating the flowering period of plants

Active Publication Date: 2020-11-27
HANGZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, their role in the regulation of flowering still needs to be further studied

Method used

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  • Application of TGA7 gene in regulation and control of flowering period of plant
  • Application of TGA7 gene in regulation and control of flowering period of plant
  • Application of TGA7 gene in regulation and control of flowering period of plant

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Obtaining of embodiment 1 homozygous mutant

[0025] 1. Obtaining Mutants

[0026] The TGA7 mutant seeds used were purchased from the Arabidopsis Resource Center (ABRC, the Arabidopsis Biological Resource Center, http: / / www.arabidopsis.org / ). Among them such as figure 1 (a) The number of tga7 is CS89835, and the construction method of the mutant is point mutation; the C in the EcoRV restriction site of the wild-type genome is mutated to T, so that the EcoRV restriction site in the genome of tga7 is deleted, so that it can be carried out Homozygous mutants were obtained by enzyme digestion after PCR reaction. The full-length gene coding (CDS) sequence of the tga7 gene is shown in SEQ ID NO.1.

[0027] 2. DNA Extraction from Arabidopsis Plant Leaves

[0028] Take an appropriate amount of seeds on wet filter paper, place them in a refrigerator at 4°C and treat them in the dark for 48 hours. After the seeds are broken and dormant, move them into the soil and place them ...

Embodiment 2

[0056] Embodiment 2 flowering time and rosette leaf number statistical analysis

[0057] Under the condition of 23°C, grow under the condition of long light (16h / 8h, light / dark) or short light (8h / 16h, light / dark) 10000Lux light conditions. 20 wild-type plants of Arabidopsis thaliana WT and tga7 plants with normal growth were selected, and the number of rosette leaves after flowering of the plants was counted.

[0058] Statistical results such as figure 2 As shown in (a-c), the flowering time of the tga7 plant was later than that of the wild type, and the number of rosette leaves was more than that of the wild type, which indicated that the TGA gene mutation in Arabidopsis plants delayed flowering. The tga7 mutant showed delayed flowering under both long-light and short-light conditions, suggesting that TGA7 may not be involved in the photoperiodic pathway.

Embodiment 3

[0059] Example 3 Expression analysis of genes related to processing of five flowering regulatory pathways in TGA mutants and wild type

[0060] 1. Processing of five flowering regulatory pathways

[0061] (1) Vernalization: Plants: Col, FRI

[0062] ①Treatment group: plant in 1 / 2MS of no-antibiotic medium, place at 4°C for 2 days to break dormancy, place in the greenhouse for 4 days, place at 4°C for 6 weeks, place in the greenhouse for 5 days, and then plant in the soil (LD / SD).

[0063] ②Control: 36 days after the start of dormancy breaking treatment in the treatment group, 1 / 2MS in the non-antibiotic medium was planted at 4°C for 2 days to break dormancy, placed in the greenhouse for 9 days, and then planted in soil (LD / SD).

[0064] ③ Sampling was taken 9 days after seedling transplantation, and the expression level of TGA7 gene was quantitatively analyzed.

[0065] (2) Gibberellin: Plants: Col, tga7

[0066] ①Treatment group: SD 100μM GA3, spray once a week from the 7t...

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Abstract

The invention discloses application of a TGA7 gene in regulation and control of a flowering period of a plant. Compared with a wild type plant, a plant with a point mutation of the TGA7 gene of arabidopsis thaliana delays flowering. A single base in the TGA7 gene of the arabidopsis thaliana is mutated. The number of lotus-base leaves of the arabidopsis thaliana plant with the mutated TGA7 gene ismore than that of the wild type plant under long-day and short-day conditions. In an autonomous pathway mutant, expression of the TGA7 in fca-2 and fv-4 is increased, and expression of the TGA7 in fld-3 and flk-1 is reduced. The expression amounts of the TGA7 gene in the wild type WT plant are sequentially and remarkably increased at the temperature of 16-27 DEG C, the expression of the TGA7 in ansvp-41 plant is remarkably reduced at the temperature of 23 DEG C, and the expression of the TGA7 in a 35S:SVP plant is remarkably increased. The expression amounts of key genes FLC, MAF5 and SMZ ina flowering regulation autonomous pathway in the arabidopsis thaliana plant with the mutated TGA7 gene are remarkably increased, and the expression amount of NF-YC2 is remarkably reduced.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular the application of TGA7 gene in regulating flowering period of plants. Background technique [0002] TGACG-Binding (TGA) transcription factors belong to the bZIP transcription factor family. The Arabidopsis TGA family has 10 members and plays important roles in plant defense and plant development. These TGAs can interact with the NPR1 gene (repressor of non-pathogenicity-related gene 1) and are involved in salicylic acid-mediated gene expression (such as PR-1) and disease resistance in Arabidopsis. These TGAs can bind to cis-regulated TGACG elements. Studies have shown that this element exists in the promoter of PR1, and PR1 gene expression requires the promoter to respond to SA and interact with NPR1. NPR1 cannot directly bind to the genomic region of PR1, and NPR1 first interacts with TGA to mediate the expression of PR1. Among the 10 TGAs in Arabidopsis, NPR1 can in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12Q1/6895
CPCC07K14/415C12Q1/6895C12Q2600/13C12Q2600/158C12Q2600/156
Inventor 秦诚徐晓蕊许静雅袁陈胡一凯刘庆港
Owner HANGZHOU NORMAL UNIVERSITY
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