Centrifugal separation device and mixed solution separation and culture method

Abstract

The invention provides a centrifugal separation device. The centrifugal separation device comprises a centrifugal tank, at least one solution guiding pipeline, at least one storage tank, at least oneswitch assembly and at least one pipette, wherein the centrifugal tank is used for centrifuging a mixed solution, and the mixed solution comprises at least two single-body solutions; one end of solution guiding pipeline communicates with an inner cavity of the centrifugal tank, and the solution guiding pipelines are used for guiding the mixed solution into the inner cavity of the centrifugal tankor guiding the mixed solution out of the inner cavity of the centrifugal tank; the storage tanks are connected with the centrifugal tank, each storage tank is provided with a connection cavity and a storage cavity, the connection cavities communicate between the storage cavities and the inner cavity of the centrifugal tank, and the storage cavities are used for storing the solutions separated fromthe mixed solution; the switch assemblies are connected with the storage tanks and used for controlling communicating or blocking of the connection cavities; and one ends of the pipettes communicatewith the storage cavities and used for guiding out of the single-body solutions in the storage cavities. The invention also provides a mixed solution separation and culture method. The centrifugal separation device can improve the separation efficiency, the purity and the stability of the mixed solution.

Description

technical field [0001] The present application relates to the technical field of centrifugal separation, in particular to a centrifugal separation device and a method for separating and cultivating a mixed liquid. Background technique [0002] Cell specific gravity separation technique is one of the most widely used cell separation techniques. At present, the current application status of similar technologies is almost done separately from cell separation, cell proliferation culture, cell washing and other technical operations, which greatly reduces the efficiency of cell preparation in vitro, and also greatly increases the possibility of isolated samples being contaminated by the outside world. . In the existing extremely limited integrated treatment technology of cell separation and cell proliferation and culture, there are many defects such as complex structure of consumables used for cell separation and culture, high standards of production materials, difficult manufact...

AI Technical Summary

Problems solved by technology

At present, the current application status of similar technologies is almost done separately from cell separation, cell proliferation culture, cell washing and other technical operations, which greatly reduces the efficiency of cell preparation in vitro, and also greatly increases the possibility of isolated samples being contaminated by the outside world.

In the existing extremely limited integrated treatment technology of cell separation and cell proliferation and culture, there are many defects such as complex structure of consumables used for cell separation and culture, high standards of production materials, difficult manufacturing process, and high production cost of products. The cell separation efficiency, Purity and stability also need to be further improved

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