Method for quantitatively analyzing relationship between plant stomata closure and root genotype by using mutual grafts

Abstract

The invention provides a method for quantitatively analyzing a relationship between plant stomata closure and root genotype by using mutual grafts. The method comprises the steps of: taking two plantsand mutual grafts thereof as analysis materials, measuring soil water content and weight of each lysimeter unit at preset regular intervals under drought conditions, and calculating instantaneous transpiration rates; taking the maximum daily transpiration rates as vertical coordinates and corresponding soil water content as horizontal coordinates, separately drawing scatter diagrams of the maximum daily transpiration rates of four materials changing with the soil water content, carrying out segmental fitting through a nonlinear curve fitting method to obtain transpiration rate change curves,wherein the soil water content corresponding to corners of the transpiration rate change curves is corresponding soil water threshold values when plant stomata are closed; and comparing the soil waterthreshold values of the four materials to judge the relationship between the plant stomata closure and the root genotype. According to the method, the relationship between the plant stomata closure and the root genotype can be quantitatively analyzed, and targeted implementation of accurate control and improvement of the drought resistance according to the characteristics of different plants is facilitated.

Description

technical field [0001] The invention relates to the technical field of plant biotechnology, in particular to the technical field of the relationship between plant stomatal closure and root genotype, and specifically refers to a method for quantitatively analyzing the relationship between plant stomatal closure and root genotype by using mutual grafts. Background technique [0002] The increasing soil drought around the world has posed a huge threat to agricultural production. Roots and leaves are the main organs of the underground and aboveground parts of the plant respectively. Plants absorb water and mineral elements through the roots, and transpiration through the stomata of the leaves creates a water potential difference between the aboveground and underground parts, maintaining the power of the root system to absorb water; stomata are also CO 2 access to the plant body. Roots and shoots interact to regulate the physiological functions of plants under different soil moi...

AI Technical Summary

Problems solved by technology

However, the current studies on the relationship between soil drought-root signal-stomatal closure are all qualitative or semi-quantitative methods, and it is still unclear whether the closure of stomata in the process of soil dehydration depends only on the signal transmitted by the root. distinguish

However, this question is of great value in production, because if it can be determined that the sensitivity of stomatal responses to drought is determined solely by root genotype, it will be possible to selectively apply regulators to plant roots to regulate stomata in agricultural production, In order to balance plant growth and drought resistance; on the contrary, it is necessary to consider the interaction between roots and shoots, and adopt more complex regulatory strategies

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