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A kind of construction method of rabies virus G protein-capeppox virus recombinant vaccine

A technology of rabies virus and sheep pox virus, which is applied in the field of medicine, can solve problems such as long cycle time, complicated procedures, and difficult promotion and use in flocks, and achieve the effect of starting transcription and translation and simplifying the construction process

Active Publication Date: 2022-06-28
TARIM UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although the current rabies vaccine can effectively prevent the occurrence of rabies, the cost of injection of rabies vaccine is high, the cycle is long, and the procedure is complicated, so it is difficult to promote the use in flocks

Method used

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  • A kind of construction method of rabies virus G protein-capeppox virus recombinant vaccine
  • A kind of construction method of rabies virus G protein-capeppox virus recombinant vaccine
  • A kind of construction method of rabies virus G protein-capeppox virus recombinant vaccine

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Embodiment Construction

[0092] The technical solutions of the present invention will be described in further detail below with reference to the embodiments.

[0093] A method for constructing a rabies virus G protein-sheeppox virus recombinant vaccine, comprising the following steps:

[0094] Step 1. Design of recombinant virus vaccine construction model

[0095] According to the design principle of poxvirus recombinant vaccine, the sheep pox virus TK gene is selected as the non-essential region for foreign gene insertion, the vaccinia virus VV7.5 and goat pox virus A8R bidirectional promoters are the alternative promoters, and the green fluorescent light protein is the reporter gene , and construct the expression vector of recombinant virus with rabies virus G protein. In order to screen the best vaccine combination, a total of 10 combinations were designed, and the G protein was selected in two forms: full-length and extra-membrane. Specific design models such as figure 1 .

[0096] (1) Acc65 I...

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Abstract

The invention discloses a method for constructing a recombinant vaccine of rabies virus G protein-oceppox virus, comprising the following steps: step 1, design of recombinant virus vaccine construction model; step 2, amplification of each gene and fusion amplification of each combined gene Step 3, construction of various combinations of recombinant transfer vectors; Step 4, recombination and virus purification of rabies virus G protein-goat pox virus; Step 5, rabies virus G protein expression detection; Step 6, rabies virus G protein-goat pox Detection of immune effect of recombinant virus vaccine strain. The vaccine constructed by the method of the invention can effectively prevent sheep rabies, has the characteristics of high production efficiency, convenience and practicality, and is suitable for popularization and application.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to a construction method of a rabies virus G protein-sheeppox virus recombinant vaccine. Background technique [0002] Rabies is a highly lethal and contagious zoonotic disease caused by the rabies virus, which can infect almost all mammals. my country is one of the countries with high incidence of rabies. In addition, the country is rich and vast, and wild animals such as wolves and foxes are more likely to carry rabies virus. Therefore, cattle and sheep in pastoral areas are attacked by wild animals and infected with rabies. , Pet dogs biting sheep and the incidence of people have also been reported. For example, Shanxi, Inner Mongolia, Xinjiang and other places have cases of cattle and sheep being bitten by dogs and infected with rabies. Sheeppox virus is a member of the Poxviridae family and is a large linear DNA virus whose genome can accommodate a large number of exogenous gen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/01C12N15/47C12N15/65C12N5/071A61K39/295A61K39/205A61K39/275A61P31/20A61P31/14
CPCC12N7/00C07K14/005C12N15/65C12N5/0683A61K39/12A61P31/20A61P31/14A61K2039/552A61K2039/5254A61K2039/70C12N2760/20122C12N2760/20134C12N2710/24221C12N2710/24234C12N2830/205
Inventor 李有文童剑军何川川张雪萍米丽开姆·托合提尼亚孜高娜杨勇飞
Owner TARIM UNIV
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