Method for constructing plant transcription factor-to-target gene genetic regulation network

Abstract

The invention relates to a method for constructing a plant transcription factor-to-target gene genetic regulation network, and belongs to the technical field of molecular genetics. The method comprises the following steps: obtaining SNP genotype data of a plant transcription factor to be detected in each individual in a group, expression quantity data of a specific tissue of the plant transcription factor to be detected in each individual in the group, and expression quantity data of a same tissue of a plant candidate target gene to be detected in each individual in the group; determining SNPsignificantly associated with the candidate target gene expression quantity; determining candidate target genes highly related to the expression quantity of the transcription factor to be detected. When the determination conditions are met at the same time, it is indicated that the transcription factor to be detected regulates the expression of the candidate target genes, and a genetic regulationnetwork of the transcription factor to the target gene can be constructed accordingly. According to the method, the regulation relationship between a plant transcription factor and downstream target genes can be accurately identified in a high-throughput manner, and a genetic regulation network of the transcription factor to the target gene is constructed.

Description

technical field [0001] The invention relates to the technical field of molecular genetics, in particular to a method for constructing a genetic regulation network of plant transcription factors on target genes. Background technique [0002] Most of the traits of plants are complex traits, which are affected by multi-level and multi-gene genetic regulation on the genome, and the genetic regulation mechanism is very complicated. Among them, the regulation of transcription level is the most important link in gene regulation. In particular, the transcriptional regulatory network can intuitively reflect the relationship between gene expression and regulation, and has become a hot issue in biological research. Studies have shown that transcription factors are key nodes in the transcriptional regulatory network, which can regulate the complex traits of plants by extensively regulating the expression of downstream genes. At present, there are two methods for identifying the regula...

AI Technical Summary

Problems solved by technology

At present, there are two methods for identifying the regulatory relationship between transcription factors and downstream genes and the construction of transcriptional regulatory networks: 1) Based on bioinformatics prediction, most of these methods only consider the sequence characteristics of the binding regions of transcription factors and downstream gene promoters. 2) Relying on molecular biology experiments, this method is time-consuming and expensive, making it difficult to achieve large-scale identification

Therefore, the existing technology lacks a high-throughput and accurate method for identifying the genetic regulatory network of plant transcription factors on downstream target genes, which increases the difficulty of analyzing and regulating the genetic regulatory network of plant complex traits

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