Application of glaucocalyxin A in preparation of product for preventing and treating periodontal disease

A technology of cyanine A and periodontal disease, applied in the field of preparation of prevention, cyanine A, and treatment of periodontal disease products, which can solve problems such as limiting the effectiveness of antibiotics

Active Publication Date: 2020-10-30
SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in recent years, antibiotic resistance has been recognized as a major risk to global health, and researchers have isolated multiple drug-resistant strains from infected pulp and periodontal tissue, suggesting that the oral ca

Method used

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  • Application of glaucocalyxin A in preparation of product for preventing and treating periodontal disease
  • Application of glaucocalyxin A in preparation of product for preventing and treating periodontal disease
  • Application of glaucocalyxin A in preparation of product for preventing and treating periodontal disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 GLA Drug Sensitivity Test to P.gingivalis

[0047] P.gingivalis was used as an experimental object to observe and study the effects of different treatments on periodontal pathogens. The frozen P.gingivalis ATCC 33277 strain was routinely inoculated and recovered in an ultra-clean bench. After successful resuscitation, take an appropriate amount of bacterial solution and spread it evenly on the BHI agar plate by the four-zone method, and put the plate upside down and put it in a constant temperature anaerobic box at 37°C for anaerobic culture for 72 hours to form uniform black colonies. Some colonies were picked for Gram staining, and the recovered strains were identified according to the morphological characteristics of P.gingivalis under an optical microscope. After the identification was correct, the colonies were picked and inoculated into BHI liquid medium for enrichment culture.

[0048] Take the P.gingivalis cultured to the logarithmic growth phase, and...

Embodiment 2

[0057] Example 2 Effect of GLA on P.gingivalis biofilm

[0058] The experimental group was divided into 0.625 μM, 1.25 μM, 2.5 μM, and 5 μM according to the final concentration of GLA. GLA was not added to the medium of the negative control group, and the specific treatment process was the same as that of the above drug sensitivity experiment. Put the configured 96-well plate into a 37°C constant temperature anaerobic box for anaerobic culture for 48 hours, then take it out, discard the excess liquid in the well plate, and gently wash it with PBS three times. Be careful and gentle during the washing process so as not to damage the biofilm. integrity. Then add methanol to the orifice plate to fix the biofilm for 15 minutes, discard the methanol and wait for the 96-well plate to dry, add 0.4% crystal violet (CV) solution to each well and stain for 15 minutes, discard the dye, and rinse the excess dye. After the 96-well plate was fully dried, add 100 μL of 95% ethanol solution t...

Embodiment 3

[0060] Example 3 Effect of GLA on P.gingivalis Biofilm Bioactivity

[0061] The grouping and treatment are the same as in Example 2. Put the configured 96-well plate into a 37°C constant temperature anaerobic box for 48 hours of anaerobic culture, then take it out, discard the excess liquid in the well plate, wash it gently with PBS three times, and then pour it into the well. Add 50 μL of 0.5% MTT to the plate, incubate in the dark for 4 hours, discard the liquid in the well, add 100 μL DMSO to dissolve, shake gently for 10 minutes, and read the OD value at a wavelength of 490 nm with a microplate reader.

[0062] from image 3 It can be seen that when the concentration of GLA is less than 1.25 μM, it has no significant inhibitory effect on the biological activity of P. gingivalis biofilm (p>0.05). When the concentration reached 1.25μM, the biological activity of P.gingivalis biofilm began to be inhibited (p<0.01), and 20% of the biofilm activity was inhibited at this time; ...

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Abstract

The invention relates to the field of periodontal disease treatment products, in particular to an application of glaucocalyxin A in preparation of products for preventing and treating periodontal diseases including gingival diseases and periodontitis. The glaucocalyxin A comprises glaucocalyxin A derivatives, natural glaucocalyxin A and soluble salts thereof. The invention also provides an application of glaucocalyxin A in inhibiting the activity of porphyromonas gingivalis. The glaucocalyxin A can be used for preparing products for preventing and treating periodontal diseases, and has no drugresistance problem.

Description

technical field [0001] The invention relates to the field of products for treating periodontal disease, in particular to the use of cyanine A in the preparation of products for preventing and treating periodontal disease. Background technique [0002] Periodontitis (periodontitis) is a microbial-related, host-mediated, multi-factorial inflammatory disease that leads to loss of periodontal attachment. Drug therapy is an adjuvant treatment for periodontitis. At present, the most commonly used clinical drugs are some antibiotics, such as metronidazole, amoxicillin, etc., which mainly inhibit certain types of bacteria and bacteria The complex exerts its adjuvant therapeutic effect. However, in recent years, antibiotic resistance has been recognized as a major risk to global health, and researchers have isolated multiple drug-resistant strains from infected pulp and periodontal tissue, suggesting that the oral cavity is likely to be a A reservoir of resistant bacteria that limi...

Claims

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Application Information

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IPC IPC(8): A61K31/122A61K36/53A61P1/02A61P31/04A23L33/105
CPCA61K31/122A61K36/53A61P1/02A61P31/04A23L33/105A23V2002/00A23V2250/21A23V2200/312
Inventor 宋忠臣周薇周鲁先杨钰婷何智妍胡苡宋莉
Owner SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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