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Method for improving solubility of dual hydrophobic protein

A hydrophobic protein and hydrophobic technology, which is applied in the preparation method of peptides, plant protein processing, chemical instruments and methods, etc., can solve the problems that the solubility of two hydrophobic proteins cannot be improved at the same time, and the primary structure and nutritional value of the protein are destroyed. Achieve the feasibility of large-scale industrial application, novel technology, and the effect of improving solubility

Pending Publication Date: 2020-10-30
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the existing reports are similar to the above-mentioned methods. Most of these methods destroy the primary structure and nutritional value of the protein, and cannot simultaneously improve the solubility of the two hydrophobic proteins.

Method used

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  • Method for improving solubility of dual hydrophobic protein
  • Method for improving solubility of dual hydrophobic protein
  • Method for improving solubility of dual hydrophobic protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A method for increasing the solubility of a doubly hydrophobic protein comprising the steps of:

[0034] (1) Alkali dispersion: put rice protein and hemp protein into distilled water at a mass ratio of 1:0.2, stir well, add alkali solution to adjust pH=10;

[0035] (2) Structure unfolding: Stir the feed solution obtained in step (1) for 60 minutes at a stirring rate of 600 r / min, so that the structures of the two proteins in the solution are fully unfolded and the secondary structure is exposed;

[0036] (3) Hydrophobic binding: Stir the feed solution obtained in step (2) for 60 min at a stirring rate of 900 r / min, and at the same time add acid solution dropwise to adjust the pH to 7.0, so that the two proteins can pass through the hydrophobic interaction between the secondary structures produce a combination;

[0037] (4) Desalination by dialysis: dialyze the feed liquid obtained in step (3) for 22 hours through a dialysis bag;

[0038] (5) centrifugation: centrifuge...

Embodiment 2

[0041] A method for increasing the solubility of a doubly hydrophobic protein comprising the steps of:

[0042] (1) Alkali dispersion: put rice protein and walnut protein into distilled water at a mass ratio of 1:0.5, stir well, add alkali solution to adjust pH=11;

[0043] (2) Structure development: Stir the feed liquid obtained in step (1) for 100 min at a stirring rate of 900 r / min, so that the structures of the two proteins in the solution are fully developed and the secondary structure is exposed;

[0044] (3) Hydrophobic binding: Stir the feed solution obtained in step (2) for 45 minutes at a stirring rate of 900 r / min, and at the same time add acid solution dropwise to adjust the pH to 7.0, so that the two proteins can pass through the hydrophobic interaction between the secondary structures Combine;

[0045] (4) Desalination by dialysis: dialyze the feed liquid obtained in step (3) for 24 hours through a dialysis bag;

[0046] (5) centrifugation: centrifuge the feed ...

Embodiment 3

[0049] A method for increasing the solubility of a doubly hydrophobic protein comprising the steps of:

[0050] (1) Alkali dispersion: Put rice protein and hemp protein into distilled water at a mass ratio of 1:1, stir well, add alkali solution to adjust pH=12;

[0051](2) Structure unfolding: Stir the feed solution obtained in step (1) for 45 minutes at a stirring rate of 1200 r / min, so that the structures of the two proteins in the solution are fully unfolded and the secondary structure is exposed;

[0052] (3) Hydrophobic binding: Stir the feed liquid obtained in step (2) at a stirring rate of 600r / min for 75min, and at the same time add acid solution dropwise to adjust the pH to 7.0, so that the two proteins can pass through the hydrophobic interaction between the secondary structures combined;

[0053] (4) Desalination by dialysis: dialyze the feed liquid obtained in step (3) for 26 hours through a dialysis bag;

[0054] (5) centrifugation: centrifuge the feed liquid ob...

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Abstract

The invention discloses a method for improving solubility of a double hydrophobic protein, and belongs to the technical field of deep processing of food proteins. The preparation method comprises thefollowing steps: dispersing two hydrophobic proteins, namely rice protein and hemp protein or walnut protein, in water, adjusting the acidity to enable the pH value to be greater than or equal to 10.0, stirring and neutralizing to synchronously solubilize the two hydrophobic proteins, dialyzing, centrifuging, and freeze-drying a supernatant to obtain the soluble hydrophobic protein compound. The protein content of the soluble rice treated by the method is increased to 85% or above, the protein content of the soluble hemp protein or walnut protein is increased to 100%, and meanwhile, the primary structures of the two proteins are completely reserved. The method is environment-friendly, economical and easy to use, completely reserves the nutrition and functional characteristics of the hydrophobic protein, and greatly expands the application range of the hydrophobic protein.

Description

technical field [0001] The invention relates to a method for improving the solubility of double hydrophobic protein, which belongs to the technical field of deep processing of food protein. Background technique [0002] With the explosive growth of the world population, the demand for protein resources is increasing day by day. However, a large number of high-quality protein resources, especially plant protein resources, have not been widely used due to their high hydrophobicity and need to be developed urgently. Wherein, in my country, rice protein is a by-product from the rice starch industry, and hemp protein is a by-product from the hemp oil industry, and the annual output of the two proteins is huge. However, due to the problem of low water solubility, the application scenarios of the two proteins are very limited, and a large amount of rice protein and hemp protein are only used as animal feed, resulting in a great waste of resources. Improving the solubility of rice...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J3/14C07K1/14
CPCA23J3/14C07K1/145
Inventor 王涛王韧黎露露冯伟陈正行李方斯
Owner JIANGNAN UNIV
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