SNP marker, detection primer pair and reagent kit for pilose antler yield traits and applications

A primer pair and deer antler technology, applied in the field of genetic biology, can solve the problems of deer antler without clear and reliable molecular markers, poor reproducibility, and SNP markers that cannot withstand verification

Pending Publication Date: 2020-10-20
长春科技学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to different research methods, environmental factors and inconsistencies in the standard definition of research samples, the obtained SNP markers cannot withstand verification, and the repeatability of the experiment is poor. Therefore, there is no clear and reliable molecular marker for velvet antler yield traits.

Method used

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  • SNP marker, detection primer pair and reagent kit for pilose antler yield traits and applications
  • SNP marker, detection primer pair and reagent kit for pilose antler yield traits and applications
  • SNP marker, detection primer pair and reagent kit for pilose antler yield traits and applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 Obtaining SNP markers related to velvet yield traits

[0051] Based on the high similarity of the genome sequences of sika deer and red deer (the comparison rate of genome sequencing data is >99%), it is obtained by systematic analysis of the red deer genome (Genbank accession number: PRJNA564362) and the sika deer genome (Genbank accession number: PRJNA541418). The steps are:

[0052] S1 takes the difference in production of the same antler during the growth period as a reference, and uses the high-quality chromosome-level red deer genome (Genbank accession number: PRJNA564362) as a reference genome to sequence the body weight of 100 sika deer individuals with significant differences in antler production, and detect the sika deer genome (Genbank accession number: PRJNA541418) data, and compared with the reference genome, obtained 39,788,029 preliminary SNP marker sites;

[0053] S2 Perform genome-wide association analysis on the obtained preliminary SNP mark...

Embodiment 2

[0067] Example 2 Verification of sika deer individuals with high antler production using five SNP markers

[0068] For the DNA fragments corresponding to the five SNP marker sites obtained in Example 1, in combination with Primer5.0, a pair of detection primers was designed to finally obtain a pair of primers as shown in SEQ ID NO: 1-10:

[0069] 1F: 5'-GTAGACTCCGGGGGTTTGTG-3' (SEQ ID NO.1),

[0070] 1R: 5'-AATTCTCCAGCAGTCCGGTG-3' (SEQ ID NO.2);

[0071] 2F: 5'-AAGTGACTACTCCCTGTCCCA-3' (SEQ ID NO.3),

[0072] 2R: 5'-CCAATCAACCCACCAACCCA-3' (SEQ ID NO.4);

[0073] 3F: 5'-CCAATCAACCCACCAACCCA-3' (SEQ ID NO.5),

[0074] 3R: 5'-TCCTAACCACTGGACCACCA-3' (SEQ ID NO.6);

[0075] 4F: 5'-GTCGGGAAAATCCCCTGGAG-3' (SEQ ID NO.7),

[0076] 4R: 5'-CCTTCGGTCCTAGGGTGAGA-3' (SEQ ID NO.8);

[0077] 5F: 5'-TGGTGATGCCATCCAACCAT-3' (SEQ ID NO.9),

[0078]5R: 5'-AATGCTGGTGGGAAAATGGC-3' (SEQ ID NO.10); After the primer design, it was synthesized by Sangon Bioengineering (Shanghai) Co., Ltd., us...

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Abstract

The invention discloses an SNP marker, detection primer pair and reagent kit for pilose antler yield traits and applications. The SNP marker comprises five SNP maker sites of S1, S2, S3, S4 and S5, and five corresponding detection primer pairs as shown in SEQID NO.1-10, a method for detecting the pilose antler yield traits is established, and the invention provides an application of the constructed primer pairs or SNP marker sites to a breeding technique and further provides the reagent kit which is constructed by the primer pair. The SNP marker, the detection primer pair and the reagent kit have the beneficial effects that through system analysis, the five SNP marker sites are sorted, and pinpoint detection is realized; and besides, the five SNP marker sites can provide new materials formolecular breeding, and provide a basis for auxiliary selection of the markers for pilose antler high-yield traits, so that multiple functions of the SNP marker sites can be realized. The method for detecting the pilose antler yield traits is adopted, so that the problem of actual production can be effectively solved, and the economic benefits are increased. Through the detection reagent kit, theoperation is simple and the precision is high.

Description

technical field [0001] The invention relates to the technical field of genetic biology, in particular to SNP markers, detection primer pairs, kits and applications of velvet antler yield traits. Background technique [0002] With its miraculous medicinal value and nourishing function, deer antler plays an irreplaceable role in the field of Chinese medicinal materials. my country is the main breeding country for antler deer in the world, but due to weak basic research, the science and technology of the deer industry has been at a plateau, without major breakthroughs, and the level of breeding is low, and it is in the stage of phenotypic selection. The key technical problems affecting the production of deer antler cannot be effectively solved, and the overall production efficiency of the industry is low, which seriously affects the stability and sustainable development of the deer industry. [0003] Breeding sika deer varieties with high antler production has always been the ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11G16B20/20G16B30/10
CPCC12Q1/6888C12Q1/6858G16B20/20G16B30/10C12Q2600/156C12Q2600/124C12Q2600/172C12Q2531/113Y02P60/87
Inventor 胡鹏飞巴恒星邓永砚李春义
Owner 长春科技学院
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