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Application of a cryopreservation solution containing peptide compounds in cryopreservation of organs and tissues

A cryopreservation solution and cryopreservation technology, applied in the field of biomedical materials, can solve the problems of affecting the survival rate of cryopreserved objects, safety function expression, inability to effectively control ice crystal growth, and lack of ice crystal growth, etc., and achieve excellent cell survival rate. , Good ice control effect, the effect of maintaining cell viability

Active Publication Date: 2022-05-27
PEKING UNIV THIRD HOSPITAL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, existing cryopreservation reagents cannot effectively control the growth of ice crystals during rewarming, which can damage cells
The high concentration (≥15%) toxic organic solvents used in the current vitrification method, such as: DMSO, lead to toxic side effects of cryopreservation reagents on cells, seriously affecting the survival rate and even the safety of cryopreservation objects after recovery sexual and functional expression
In summary, the cryopreservation reagents currently used do not have the ability to effectively control the growth of ice crystals during the rewarming process, and there is also the problem of poor reagent safety.

Method used

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  • Application of a cryopreservation solution containing peptide compounds in cryopreservation of organs and tissues
  • Application of a cryopreservation solution containing peptide compounds in cryopreservation of organs and tissues
  • Application of a cryopreservation solution containing peptide compounds in cryopreservation of organs and tissues

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preparation example Construction

[0084] The preparation method of the present invention will be described in further detail below with reference to specific examples. It should be understood that the following examples are only for illustrating and explaining the present invention, and should not be construed as limiting the protection scope of the present invention. All technologies implemented based on the above content of the present invention are covered within the intended protection scope of the present invention.

[0085] The experimental methods used in the following examples are conventional methods unless otherwise specified; the reagents, materials, etc. used in the following examples can be obtained from commercial sources unless otherwise specified.

Embodiment 1

[0086] Example 1 Synthesis of compound of formula (1)

[0087] (1) Put 2-Chlorotrityl Chloride Resin (2-Chlorotrityl Chloride Resin) into a reaction tube, add DCM (20 mL g -1 ), shake for 30 minutes. Sand core suction filtration to remove the solvent, add three times molar excess of Fmoc-L-Pro-OH, then add 8 times molar excess of DIEA, and finally add DMF to dissolve, and shake for 30 minutes. Methanol cap for 30 minutes.

[0088] (2) Remove the solvent DMF, add 20% piperidine / DMF solution (10 mL g -1 ), after 5 minutes the solvent was removed and 20% piperidine / DMF solution (10 mL g -1 ), the piperidine solution was removed after 15 minutes. Take a small amount of resin, wash it with ethanol three times, add ninhydrin reagent, heat at 105-110 °C for 5 minutes, and turn dark blue as a positive reaction.

[0089] (3) above-mentioned reaction obtains product with DMF (15mL g -1 , twice), methanol (15mLg -1 , twice) and DMF (15mL g -1 , twice) after washing, Fmoc-L-Thr(tB...

Embodiment 2

[0095] Example 2 Synthesis of compound of formula (2)

[0096] (1) put 2-chlorotrityl chloride resin into the reaction tube, add DCM (20mL g -1 ), shake for 30 minutes. Sand core suction filtration to remove the solvent, add three times molar excess of Fmoc-L-Thr(tBu)-OH, then add 8 times molar excess of DIEA, finally add DMF to dissolve, and shake for 30 minutes. Methanol cap for 30 minutes.

[0097] (2) Remove the solvent DMF, add 20% piperidine / DMF solution (10 mL g -1 ), after 5 minutes the solvent was removed and 20% piperidine / DMF solution (10 mL g -1 ), the piperidine solution was removed after 15 minutes. Take a small amount of resin, wash it with ethanol three times, add ninhydrin reagent, heat at 105-110 °C for 5 minutes, and turn dark blue as a positive reaction.

[0098] (3) above-mentioned reaction obtains product with DMF (15mL g -1 , twice), methanol (15mLg -1 , twice) and DMF (15mL g -1 , twice) after washing, Fmoc-Arg(Pbf)-OH dissolved with as little D...

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Abstract

The invention discloses the application of a cryopreservation solution containing peptide compounds in organ and / or tissue cryopreservation. The peptide compound is composed of ice-loving amino acids and hydrophilic groups. It has good ability to inhibit the growth of ice crystals and modify the shape of ice crystals in aqueous solution, and has no thermal hysteresis and good biocompatibility. It is an ideal ice control material. . The cryopreservation solution containing the above-mentioned peptide compound contains the above-mentioned peptide compound, polyols, water-soluble sugar and other components, and can maintain the complete follicular structure when used for cryopreservation of ovarian tissues or organs. The cryopreservation solution has simple components and stable performance.

Description

[0001] This application claims the priority of the prior application with the patent application number 201910281986.7 and the invention title "A peptide compound and a cryopreservation solution containing the compound" filed with the State Intellectual Property Office of China on April 9, 2019. The entire contents of this prior application are incorporated herein by reference. technical field [0002] The invention belongs to the technical field of biomedical materials, in particular to the application of a cryopreservation solution containing a peptide compound in the cryopreservation of organs and tissues. Background technique [0003] Cryopreservation refers to the preservation of biological materials under ultra-low temperature, which slows down or stops cell metabolism and division, and can continue to develop once the normal physiological temperature is restored. Since its inception, this technology has become one of the indispensable research methods in the field of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K5/062C07K5/068C07K5/078C07K5/083A01N1/02
CPCC07K5/0606C07K5/06095C07K5/06165C07K5/0806C07K5/081A01N1/0221
Inventor 严杰乔杰闫丽盈李蓉王健君金晟琳
Owner PEKING UNIV THIRD HOSPITAL
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