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Peptide compound and cryopreservation solution containing same

A technology of cryopreservation liquid and peptide compounds, which is applied in the preservation of human or animal body, peptides, animal husbandry, etc., can solve the problems of lack of ice crystal growth, inability to effectively control ice crystal growth, damage to cells, etc.

Active Publication Date: 2020-10-20
INST OF CHEM CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, existing cryopreservation reagents cannot effectively control the growth of ice crystals during rewarming, which can damage cells
The high concentration (≥15%) toxic organic solvents used in the current vitrification method, such as: DMSO, lead to toxic side effects of cryopreservation reagents on cells, seriously affecting the survival rate and even the safety of cryopreservation objects after recovery sexual and functional expression
In summary, the cryopreservation reagents currently used do not have the ability to effectively control the growth of ice crystals during the rewarming process, and there is also the problem of poor reagent safety.

Method used

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  • Peptide compound and cryopreservation solution containing same
  • Peptide compound and cryopreservation solution containing same
  • Peptide compound and cryopreservation solution containing same

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preparation example Construction

[0079] The preparation method of the present invention will be further described in detail in conjunction with specific examples below. It should be understood that the following examples are only for illustrating and explaining the present invention, and should not be construed as limiting the protection scope of the present invention. All technologies realized based on the above contents of the present invention are covered within the scope of protection intended by the present invention.

[0080] The experimental methods used in the following examples are conventional methods unless otherwise specified; the reagents and materials used in the following examples can be obtained from commercial sources unless otherwise specified.

Embodiment 1

[0081] Synthesis of embodiment 1 formula (1) compound

[0082] (1) Put 2-chlorotrityl chloride resin (2-Chlorotrityl Chloride Resin) into the reaction tube, add DCM (20mL g -1 ), shaking for 30 minutes. The sand core was filtered to remove the solvent, and a three-fold molar excess of Fmoc-L-Pro-OH was added, followed by an eight-fold molar excess of DIEA, and finally DMF was added to dissolve, and shaken for 30 minutes. Methanol capping for 30 minutes.

[0083] (2) Remove the solvent DMF, add 20% piperidine / DMF solution (10mL g -1 ), after 5 minutes the solvent was removed, and a 20% piperidine / DMF solution (10 mL g -1 ), the piperidine solution was removed after 15 minutes. Take a small amount of resin, wash it three times with ethanol, add ninhydrin reagent, heat at 105-110°C for 5 minutes, if it turns dark blue, it is a positive reaction.

[0084] (3) The product obtained by the above reaction was washed with DMF (15mL g -1 , twice), methanol (15mL g -1 , twice) and...

Embodiment 2

[0090] Synthesis of embodiment 2 formula (2) compound

[0091] (1) Put 2-chlorotrityl chloride resin into the reaction tube, add DCM (20mL g -1 ), shaking for 30 minutes. The sand core was filtered to remove the solvent, and a three-fold molar excess of Fmoc-L-Thr(tBu)-OH was added, followed by an eight-fold molar excess of DIEA, and finally DMF was added to dissolve, and shaken for 30 minutes. Methanol capping for 30 minutes.

[0092] (2) Remove the solvent DMF, add 20% piperidine / DMF solution (10mL g -1 ), after 5 minutes the solvent was removed, and a 20% piperidine / DMF solution (10 mL g -1 ), the piperidine solution was removed after 15 minutes. Take a small amount of resin, wash it three times with ethanol, add ninhydrin reagent, heat at 105-110°C for 5 minutes, if it turns dark blue, it is a positive reaction.

[0093] (3) The product obtained by the above reaction was washed with DMF (15mL g -1 , twice), methanol (15mL g -1 , twice) and DMF (15mL g -1 , twice) a...

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Abstract

The invention discloses a peptide compound and a cryopreservation solution containing the same. The peptide compound is composed of ice-philic amino acid and a hydrophilic group, has good capabilitiesof inhibiting ice crystal growth and modifying ice crystal morphology when in an aqueous solution, has no thermal hysteresis, has good biocompatibility, and is an ideal ice control material. The cryopreservation solution containing the peptide compound contains the peptide compound, polyhydric alcohol, water-soluble sugar and other components, can keep a good survival rate when being used for preserving embryos or oocytes, and is simple in components and stable in performance.

Description

[0001] This application claims the priority of the previous application filed with the State Intellectual Property Office of China on April 9, 2019 with the patent application number 201910281986.7 and the title of the invention "A Peptide Compound and a Cryopreservation Solution Containing the Compound". The entirety of this prior application is incorporated by reference into the present application. technical field [0002] The invention belongs to the technical field of biomedical materials, and in particular relates to a peptide compound and a cryopreservation solution containing the compound. Background technique [0003] Cryopreservation refers to the preservation of biological materials at ultra-low temperature, which slows down or stops the metabolism and division of cells, and can continue to develop once the normal physiological temperature is restored. Since the technology came out, it has become one of the indispensable research methods in the field of natural sc...

Claims

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Application Information

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IPC IPC(8): C07K5/062C07K5/068C07K5/078C07K5/083A01N1/02
CPCC07K5/0606C07K5/06095C07K5/06165C07K5/0806C07K5/081A01N1/0221
Inventor 王健君金晟琳严杰乔杰闫丽盈李蓉
Owner INST OF CHEM CHINESE ACAD OF SCI
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