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A method for improving the quality of pig sperm after thawing

A pig sperm quality technology, applied in the preservation, application, and animal husbandry of human or animal bodies, can solve the problems of unclear internal damage mechanism, achieve the effects of avoiding loss, reducing damage, and improving centrifugation effect

Active Publication Date: 2021-12-21
JILIN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In recent years, the existing technology has conducted detailed research on the external conditions that affect the quality of cryopreserved sperm, and the external conditions can be controlled to an appropriate range during the cryopreservation process to minimize the impact of external conditions; however, the internal damage mechanism However, it is not clear how to avoid the quality loss of pig spermatozoa during the freezing process to the greatest extent has become a technical problem to be solved

Method used

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  • A method for improving the quality of pig sperm after thawing

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Embodiment 1

[0031] A method for improving the quality of pig sperm after thawing, comprising the following steps:

[0032] (1) After the collected qualified semen is diluted with semen protection diluent within 2 hours, it is placed in a 25°C incubator for 1 hour, during which the semen is mixed from time to time, and then placed in a 17°C incubator for static equilibrium treatment 2 hours, mixing at irregular times during the period, and the constant temperature equilibrium treatment is completed; the qualified semen is selected by the following method: select the semen with a slight fishy smell and milky white for microscopic examination, and the semen with an activity of more than 90% and a density between 200 and 400 million / mL For qualified semen, the semen protection diluent is made of the following components by weight: 1.25 parts of ethylenediaminetetraacetic acid, 1.25 parts of sodium citrate dihydrate, 0.75 parts of potassium chloride, 1.25 parts of sodium bicarbonate, 36.50 part...

Embodiment 2

[0037] A method for improving the quality of pig sperm after thawing, comprising the following steps:

[0038] (1) After the collected qualified semen is diluted with semen protection diluent within 2 hours, it is placed in a 25°C incubator for 1 hour, during which the semen is mixed from time to time, and then placed in a 17°C incubator for static equilibrium treatment 2 hours, mixing at irregular times during the period, and the constant temperature equilibrium treatment is completed; the qualified semen is selected by the following method: select the semen with a slight fishy smell and milky white for microscopic examination, and the semen with an activity of more than 90% and a density between 200 and 400 million / mL For qualified semen, the semen protection diluent is made of the following components by weight: 1.20 parts of ethylenediaminetetraacetic acid, 1.20 parts of sodium citrate dihydrate, 0.70 parts of potassium chloride, 1.20 parts of sodium bicarbonate, 36.00 part...

Embodiment 3

[0043] A method for improving the quality of pig sperm after thawing, comprising the following steps:

[0044] (1) After the collected qualified semen is diluted with semen protection diluent within 2 hours, it is placed in a 25°C incubator for 1 hour, during which the semen is mixed from time to time, and then placed in a 17°C incubator for static equilibrium treatment 2 hours, mixing at irregular times during the period, and the constant temperature equilibrium treatment is completed; the qualified semen is selected by the following method: select the semen with a slight fishy smell and milky white for microscopic examination, and the semen with an activity of more than 90% and a density between 200 and 400 million / mL For qualified semen, the semen protection diluent is made of the following components by weight: 1.30 parts of ethylenediaminetetraacetic acid, 1.30 parts of sodium citrate dihydrate, 0.80 parts of potassium chloride, 1.30 parts of sodium bicarbonate, 37.00 part...

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Abstract

The invention discloses a method for improving the quality of thawed pig sperm, which comprises the following steps: (1) diluting the collected qualified semen with a semen protection diluent, and performing constant temperature equilibrium treatment; (2) centrifuging the semen to obtain Add the frozen base solution Ⅰ to the centrifuged sediment, resuspend it and put it in the incubator for constant temperature equilibrium treatment; (3) add the frozen base solution Ⅱ to it, mix well, fill it in a thin tube, seal it, and fumigate it on liquid nitrogen or Use a program freezer to freeze, and then store in liquid nitrogen; (4) When the semen is thawed, take out the thin tube from the liquid nitrogen, incubate in a water bath, and then add the thawing diluent, and the thawing is complete. The present invention can effectively improve the motility, plasma membrane integrity rate and acrosome integrity of porcine sperm after thawing by jointly adding corresponding concentrations of caffeic acid phenethyl ester and hydroxyethyl starch to the freezing base liquid I. rate, DNA integrity rate, and improve the quality of pig sperm after freezing.

Description

technical field [0001] The invention relates to the technical field of cryopreservation of pig sperm, in particular to a method for improving the quality of pig sperm after thawing. Background technique [0002] With the rapid rise of pig artificial insemination technology, most pig farmers have adopted this method to breed piglets in order to reduce the cost of boar breeding. The cryopreservation of semen can make the use of semen not limited by time, place and object of use, and significantly increase the use time and utilization rate of the semen of good boars. These advantages make artificial insemination technology more popular. Therefore, frozen semen technology plays an important role. [0003] The cryopreservation of semen can effectively preserve the genetic breeding and germplasm resources of organisms. With the rapid development of pig frozen semen production technology and artificial insemination technology, the conception rate and number of piglets of breeding...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221
Inventor 李兆华张志彬钱芙蓉菅芯蕊王珺于永生昭日格图王俊恒李彦春刘向光张鑫金庆国柳海星高见红孟庆祝丁德李闯吴德忠李淑梅张志民张健
Owner JILIN ACAD OF AGRI SCI
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