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Rice low-temperature induced expression promoter Poscold10 and application thereof

A low-temperature induction and promoter technology, applied in the fields of biotechnology and plant genetic engineering, can solve the problems of developmental delay, waste of material and energy, and short plant, and achieve the effects of increasing expression, improving growth characteristics, and improving growth characteristics

Active Publication Date: 2020-10-13
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the current genetic engineering operations, constitutive promoters are often used to drive the overexpression of these key node genes. Although the obtained transgenic plants can show strong low temperature tolerance, the unnecessary expression brought about by the constitutive promoters often leads to plant failure. Short stature, developmental delay and waste of material and energy are not conducive to potential practical application promotion

Method used

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  • Rice low-temperature induced expression promoter Poscold10 and application thereof
  • Rice low-temperature induced expression promoter Poscold10 and application thereof
  • Rice low-temperature induced expression promoter Poscold10 and application thereof

Examples

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Embodiment Construction

[0026] The present invention will be described below with reference to specific examples. Those skilled in the art can understand that these examples are only for illustrating the present invention, and they do not limit the scope of the present invention in any way.

[0027] The experimental methods in the following examples are conventional methods unless otherwise specified. The biochemical reagents and consumable raw materials used in the following examples are all commercially available products unless otherwise specified.

[0028] Obtaining of Poscold10 promoter containing restriction site

[0029] Step 1. Design of primers

[0030] During the research, the applicant accidentally discovered a gene sequence with low-temperature induction properties, which was named Poscold10. For the specific sequence, please refer to the sequence listing. The applicant designed the amplification primers based on the sequence of rice Poscold10, and designed the enzyme cutting sites of ...

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Abstract

The invention provides a plant low-temperature induced expression promoter Poscold10 and application thereof. The invention further provides an expression cassette, a plant expression vector, host bacteria and a transformant containing the promoter. Specifically, the plant low-temperature induced expression promoter Poscold10 is applied in plant genetic engineering. The promoter can specifically drive the high-intensity expression of foreign genes in plants under low temperature conditions. By connecting the promoter to a gene to be expressed and transferred into a plant using a vector, the expression of the foreign gene in various parts of the plant under low temperature conditions can be driven.

Description

technical field [0001] The invention relates to the technical fields of biotechnology and plant genetic engineering. Specifically, the present invention relates to a plant low-temperature-induced expression promoter and its application. The promoter can drive the expression of a target gene in a plant in a rice transgene regulation system. Background technique [0002] Rice is one of the most important food crops in the world. In recent years, due to the El Niño phenomenon, rice has encountered extreme weather such as drought or flood during its growth stage, which has seriously affected the yield of rice. Improving the resistance of crops to extreme climates through plant genetic engineering has an important application prospect. The regulation of gene expression by promoters suggests that we can change or add promoters through genetic engineering to activate the expression of stress-resistant genes and improve the stress resistance of crops. [0003] Low temperature is a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12N15/84A01H5/00A01H6/46
CPCC12N15/8237C12N15/8205C12N15/8273
Inventor 许蓉芳李娟秦瑞英刘小双廖圣祥
Owner RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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