Screening and application of efficient straw cellulose decomposition bacteria

Abstract

The invention discloses screening of efficient straw cellulose decomposition bacteria. Screening of the efficient straw cellulose decomposition bacteria comprises the following steps: (1) conducting preliminary screening on the cellulose decomposition bacteria by adopting a sodium carboxymethylcellulose plate method, and preliminarily measuring the cellulose decomposition capability of the strainsthrough a Congo red staining method and filter paper decomposition rates; (2) measuring the filter paper enzyme activity and the carboxymethyl cellulose enzyme activity of the strains subjected to preliminary screening to continue judging the cellulose decomposition capability; and (3) identifying the cellulose decomposition bacteria, and optimizing enzyme-producing conditions for the strains which highly produce cellulase. According to screening of the efficient straw cellulose decomposition bacteria, the cellulose decomposition bacteria are separated from Dabieshan yellow cattle rumen fluid, are free from bio-safety problems, and have a good effect of fermenting corn straw; through separation and screening in a laboratory, the obtained decomposition bacteria can resist different temperatures and acid and alkali environments with high enzyme activity maintained; and clinical experiments prove that the cellulose decomposition bacteria can effectively promote fermentation of the corn straw, improves a reducing sugar content and a protein content, enhances the palatability, and is easy to digest and absorb.

Description

Technical field: [0001] The invention relates to the field of biology, in particular to the screening and application of high-efficiency straw cellulolytic bacteria. Background technique: [0002] With the rapid development of my country's animal husbandry, although great achievements have been made, it is facing the situation of human and livestock competing for food, which makes the food problem severe. If the structure of animal husbandry is not adjusted in time to reduce the dependence of animal husbandry on grain, the future development will be greatly restricted. At present, there are many research reports on using crop stalks as feed to solve the problem of livestock grain. [0003] The use of microbial technology to treat crop straw is currently the most researched method of straw treatment. A type of cellulolytic bacteria in nature can decompose cellulose such as straw into sugars, and at the same time, the bacteria itself is a very good protein, that is, single-c...

AI Technical Summary

Problems solved by technology

The premise of this application prospect is to first isolate microbial strains that can effectively decompose cellulose, but the cellulase obtained at present, whether it is from animals, plants or microorganisms, cannot meet the needs of large-scale industrial production.

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