A kind of water-soluble β-glucan and its preparation method and its application in the preparation of immunity-enhancing and anti-tumor drugs and health care products

A glucan and water-soluble technology, applied in the field of biochemistry, can solve the problem of insoluble β-glucan being difficult to degrade, and achieve the effect of facilitating industrial scale-up, expanding the scope of application, and improving phagocytosis

Active Publication Date: 2021-10-15
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to solve the problem that insoluble β-glucan is difficult to degrade, the object of the present invention is to provide a water-soluble β-glucan and its preparation method and its application in the preparation of immune-enhancing and anti-tumor drugs and health products, In the present invention, the insoluble β-glucan is first dissolved by a low-temperature strong alkali urea solution, and then degraded into water-soluble β-glucan by using hydrogen peroxide

Method used

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  • A kind of water-soluble β-glucan and its preparation method and its application in the preparation of immunity-enhancing and anti-tumor drugs and health care products
  • A kind of water-soluble β-glucan and its preparation method and its application in the preparation of immunity-enhancing and anti-tumor drugs and health care products
  • A kind of water-soluble β-glucan and its preparation method and its application in the preparation of immunity-enhancing and anti-tumor drugs and health care products

Examples

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Effect test

Embodiment 1

[0036] In this example, brewer's yeast is used as raw material to extract insoluble yeast β-glucan, and water-soluble yeast β-glucan is prepared according to the technical scheme of the present invention. The specific steps are as follows:

[0037] (1) Extraction of yeast β-glucan: Take fresh beer yeast sludge, wash it in 100 mesh gauze to remove water-soluble components, centrifuge the sediment (5000r / min) for 10min, wash it with deionized water three times, and collect the sediment . Add 1M sodium acetate aqueous solution to the precipitate at a mass ratio of 1:10, adjust the pH to 5.5 with acetic acid, then add 3% sodium chloride, stir at 55°C for 24 hours, and neutralize to pH with 2M sodium hydroxide solution Centrifuge (5000r / min) for 10min after 7 to collect the precipitate, add 0.5M sodium hydroxide solution at a mass ratio of 1:10, heat and stir at 80°C for 2h, centrifuge (5000r / min) for 10min, collect the precipitate, and wash with deionized water Three times, the y...

Embodiment 2

[0044] The preparation method of water-soluble β-glucan in this embodiment comprises the following steps:

[0045] (1) Prepare sodium hydroxide urea aqueous solution: wherein sodium hydroxide accounts for 8% of the total weight of the solution, urea accounts for 12% of the total weight of the solution, and the solution is frozen in a -20° C. refrigerator for 10 h before use.

[0046] (2) Dissolution of yeast β-glucan: Weigh 0.24g of brewer’s yeast β-glucan and add it to 88mL of the sodium hydroxide urea aqueous solution, stir in an ice bath until there is no precipitation, and then place it in a -20°C refrigerator Freeze for 24 hours to obtain a transparent and homogeneous brewer's yeast β-glucan solution.

[0047] (3) Preparation of water-soluble yeast β-glucan: heat the frozen β-glucan solution to 70°C, slowly add 8 mL of 30% aqueous hydrogen peroxide solution dropwise until the final concentration of hydrogen peroxide is 2.4%, The polysaccharide concentration is 0.25%, and...

Embodiment 3

[0050] In this example, β-glucan derived from euglena is extracted and water-soluble β-glucan is prepared, and the specific steps are as follows:

[0051] (1) Extraction of euglena β-glucan: euglena powder was refluxed and degreased three times with ethanol, centrifuged to collect the precipitate, added 0.5M potassium hydroxide solution at a mass ratio of 1:10, heated and stirred at 80°C for 2h, centrifuged ( 5000r / min, 10min), collect the clear liquid, collect the precipitate after neutralizing with hydrochloric acid, wash the precipitate three times with deionized water, then wash with ethanol and acetone in sequence, and dry to obtain insoluble euglena β-glucan, whose molecular weight is greater than 500kDa, the polysaccharide is mainly β-(1,3)-glucan, and its chemical structure is as follows:

[0052]

[0053] (2) Prepare an aqueous solution of sodium hydroxide and potassium urea, wherein potassium hydroxide accounts for 7% of the total weight of the solution, and urea ...

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Abstract

The invention discloses a water-soluble β-glucan, its preparation method and its application in the preparation of immune-enhancing and anti-tumor medicines and health care products. The present invention first prepares alkaline urea aqueous solution and freezes it, then adds insoluble β-glucan, freezes after stirring and dissolving, then heats to an appropriate temperature, adds hydrogen peroxide aqueous solution dropwise under stirring, adjusts pH to neutrality, and performs dialysis. The dialyzed internal fluid is concentrated under reduced pressure and freeze-dried to obtain water-soluble β-glucan. The method solves the technical problem that the insoluble β-glucan is difficult to degrade through the homogeneous degradation in the alkaline urea aqueous solution. The water-soluble β-glucan obtained by using the method can significantly enhance the immune activity of macrophage RAW 264.7 and effectively antagonize tumor cells. The β-glucan prepared by the present invention has the advantages of easy-to-obtain raw materials, simple preparation process, strong biological activity, and easy industrial production, and has the prospect of being developed as an immune-enhancing and anti-tumor drug.

Description

technical field [0001] The invention belongs to the technical field of biochemistry, and in particular relates to a water-soluble beta-glucan, a preparation method thereof, and an application in preparation of immune-enhancing and anti-tumor drugs and health care products. Background technique [0002] β-glucan is a class of polysaccharides composed of β-configured glucose residues in different ways, including linear β-1,3-glucan, linear β-1,4-glucan, linear β -1,6-glucan, linear β-1,3 and β-1,4-glucan in mixed arrangement, and β-1,3 / 1,4-glucan and β-glucan with branched structure 1,3 / 1,6-glucan, etc., β-glucan compounds widely exist in nature, and β-glucans with different structures have different biological activities. For example, β-1,3-glucan and β-1,6-glucan can play an anti-tumor effect by activating macrophages, natural killer cells, etc., or play an anti-inflammatory and anti-viral role by enhancing immune activity , can also be used as dietary fiber, can inhibit t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/02A61K31/716A61P37/04A61P35/00A23L33/125
CPCA23V2002/00A61K31/716A23L33/125A61P35/00A61P37/04C08B37/0024A23V2200/324A23V2200/308A23V2250/5034
Inventor 于广利王珊珊蔡超李国云郝杰杰
Owner OCEAN UNIV OF CHINA
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