A vertical microbial detection chip

Abstract

The invention relates to a vertical microbial detection chip, which comprises a conveying channel bent and extended between the inlet and the outlet of the chip and an accommodating cavity arranged at the horizontal part of the conveying channel, and an outlet is arranged at the bottom of the accommodating cavity to communicate with the next stage The side flow channel of the distribution channel arranged horizontally, the chip arranged vertically allows the excitation light source and the fluorescence detection element to be arranged on both sides of the chip, so that the background interference of the excitation light can be completely shielded; the inlet and outlet ends of the side flow channel are respectively connected to The bottom of the chamber and the lower-level distribution channel can realize the induction of micro-droplets without consuming the continuous phase fluid; the side flow channel can be set separately in the structural layer, thereby reducing or even eliminating the impact of the filled chamber on the droplet. Inductive effect; by coating a polarizing coating with opposite polarization directions on the outside of the chip cover layer and the substrate layer, the optical path between the excitation light and the fluorescence detection element is completely blocked, and at the same time, it is matched on both sides of the chip The provided external polarizing element can also block the optical path from the fluorescence signal emitted through the side wall of the accommodation cavity to the fluorescence detection element, so as to avoid signal fusion between adjacent fluorescence signal points.

Description

technical field [0001] The invention relates to a biological detection device, in particular to a vertical microbiological detection chip for performing specific DNA detection of microorganisms such as viruses and bacteria through PCR reactions. Background technique [0002] The digital PCR chip based on micro-droplets is one of the most advanced nucleic acid quantitative detection methods. Compared with traditional PCR technology, digital PCR dilutes the solution containing target genes, primers, polymerases, etc., and divides them into dozens to dozens Tens of thousands of tiny, independent reactors, so that the number of nucleic acid templates in each reactor is less than or equal to 1, and each reactor is subjected to traditional PCR amplification and fluorescence detection. The reactor containing the target gene is marked as 1, and the reactor without the target gene is marked as 0. According to the relative ratio and the volume of the reactor, the nucleic acid concentr...

AI Technical Summary

Problems solved by technology

However, due to the resistance of the microchannel itself and the local barrier, there is a large pressure difference in the continuous phase fluid flowing between the upstream and downstream of the distribution channel, which will lead to a huge difference in the filling effect of the micro-reaction chamber at the upstream and downstream of the distribution channel.

[0005] In addition, in the process of fluorescence detection of the amplified micro-reaction chamber, the reflection and refraction of the incident excitation light often cause strong background interference; Due to the non-directional nature of the fluorescence emitted by the stimulated micro-droplets in the reaction chamber, some of the fluorescence signals between adjacent micro-wells often pass through the hole wall and are mixed with the fluorescent signals from adjacent droplets, making it difficult to distinguish The problem

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