A device for cell pyrolysis using surface acoustic wave sonothermal effect

Abstract

A device for cell pyrolysis using surface acoustic wave acoustothermal effect, including a microfluidic chip, a high-speed camera and a microscope are arranged above the microfluidic chip, and the signal input of the microfluidic chip is connected to the output of a power amplifier and a signal generator. The electrical signal is generated by the signal generator, and the electrical signal is amplified by the power amplifier and transmitted to the microfluidic chip to realize the generation and control of the surface acoustic wave; there are liquid droplets suspended in the flow channel, and the cells are wrapped in the liquid droplets, and the lysis After the completion, the nucleic acid is wrapped in the droplet; the present invention uses the surface acoustic wave microfluidic device to control the temperature in the microfluidic channel, and further realizes the rapid and effective lysis of cells, and has the advantages of small equipment size, simple operation, and small clean space required ; At the same time, it has the advantages of high compatibility with various drugs or reagents, and low requirements for experimental environment and personnel.

Description

technical field [0001] The invention belongs to the technical field of microfluidic chips, and in particular relates to a device for thermally cracking cells by utilizing the acoustothermal effect of a surface acoustic wave (SAW). Background technique [0002] Cell lysis is an important method for nucleic acid detection and organelle research in biomedical experiments. With the continuous development of microfluidic chip technology, it is gradually becoming possible to use microfluidic chips to provide simple, fast and practical lysis methods for cell lysis. [0003] Currently, cell lysis is mainly achieved by chemical or physical methods. Chemical lysis is the use of lysis buffer or biological enzymes to dissolve lipids and proteins in cell membranes. Although it is simple to implement, the remaining chemical reagents will affect subsequent extraction and amplification. Physical lysis, the tearing or piercing of cell membranes by some physical action, including electropora...

AI Technical Summary

Problems solved by technology

However, in existing studies, surface acoustic wave cell lysis uses the liquid flow driving ability of surface acoustic waves to accelerate the flow of fluid media, so that cells continuously collide with micropillars, microspheres and other structures in the flow channel to achieve cell lysis, and does not involve surface acoustic waves. Thermal effect

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