Culture pore plate for drug concentration screening multi-cell co-culture and using method thereof

Abstract

The invention relates to a culture pore plate for drug concentration screening multi-cell co-culture and a using method thereof. An upper-layer cell culture area is arranged on the front surface of acore plate, and a lower-layer cell culture area and a concentration gradient generation area are arranged on the back surface; upper-layer cell culture holes are formed in the upper-layer cell culturearea; lower-layer cell culture holes are formed in the lower-layer cell culture area; the upper-layer cell culture holes and the lower-layer cell culture holes correspond to each other in position and are separated by a liquid-permeable membrane; the upstream ends of the concentration gradient forming channels are communicated with a first liquid inlet and a second liquid inlet, and the downstream ends of the concentration gradient forming channels are communicated with the lower-layer cell culture holes of the corresponding concentration gradient levels; a liquid outlet is formed in the coreplate; the tail ends of the lower-layer cell culture holes are communicated with a liquid outlet; and the lower-layer cell culture area and the concentration gradient generation area on the back surface of the core plate are covered and sealed through a liquid sealing film. According to the invention, construction of an in-vitro multi-(more than two)-cell co-culture model can be realized, perfusion culture of cells can be realized, and gradient distribution of drug concentration is realized so as to improve drug concentration screening efficiency.

Description

technical field [0001] The invention belongs to the technical field of cell culture equipment, in particular to a culture hole plate for drug concentration screening multi-cell co-cultivation and a use method thereof. Background technique [0002] The treatment of cancer is usually accompanied by the use of drugs. Usually, the use of drugs requires a large number of cell experiments and animal model experiments before they can be used in clinical human experiments. This stage takes a long time and requires a lot of financial resources. The development of clinical new drugs needs to go through a long process of several years or even decades. Therefore, the development of more effective in vitro screening drug models has become a key way to shorten the time of drug development and improve the efficiency of drug development. [0003] Traditional in vitro cell research models are mostly based on monolayer culture of cancer cells. Although this monolayer culture cell model can r...

AI Technical Summary

Problems solved by technology

[0006] Most of the existing cell culture platforms are static culture, which cannot provide fresh nutrients for cell growth in time, nor can it provide shear force for cell growth, and the use of perfusion culture is an effective way to increase animal cell density

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