Disc type microfluidic fluorescence immunoassay analyzer and immune item testing method

Abstract

The invention discloses a disc-type microfluidic fluorescence immunoassay analyzer and an immune item test method, and solves the technical problem that a detection instrument in the prior art cannotbe matched with a disc-type microfluidic fluorescence immunoassay detection chip for multi-item joint inspection by one-time sample adding to perform immune item detection. The immunoassay analyzer mainly comprises an analyzer main body and an analyzer movement. The immune item test method mainly comprises the following steps: the immune detection chip is put into the analyzer core, and differentspeeds are selected through the BLDC brushless motor; through centrifugation and capillary action, reagents and samples in the chip are uniformly mixed, transferred and reacted, and LED signal flickering and photoelectric conversion in the light path detection module are matched to complete detection. The analyzer is simple in structure, scientific and reasonable in design and convenient to use, can be accurately matched with an immunoassay chip so as to enable a reagent sample in the immunoassay chip to react and perform immunoassay item detection on the reacted reagent sample, and is high indetection efficiency and detection accuracy.

Description

technical field [0001] The invention belongs to the technical field of medical devices, and in particular relates to a dish-type microfluidic fluorescent immune analyzer and a method for testing immune items. Background technique [0002] The microfluidic reagent plate is a system that manipulates the flow of a small volume of fluid in a small channel. It can realize the preparation of samples and reagents; reaction; separation, etc. through capillary action and centrifugation. [0003] Immunofluorescence cytochemistry is based on the principle of antigen-antibody reaction. Firstly, the known antigen or antibody is labeled with fluorescein to make a fluorescent marker, and then this fluorescent antibody (or antigen) is used as a molecular probe to examine the cells or tissues. Corresponding antigen (or antibody). The antigen-antibody complex formed in cells or tissues contains fluorescein, which is irradiated with excitation light. The fluorescein emits bright fluorescence ...

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is: to provide a disc microfluidic fluorescence immunoassay analyzer and a test method for immune items, so as to solve the problem that the prior art detection instrument cannot cooperate with the disc microfluidic fluorescence immunoassay chip for multi-item joint inspection at one time. Technical Problems in Immunization Project Detection

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