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Preparation method and application of EPA/ARA type acetal phospholipid

A technology of plasmalogen and enzymatic phospholipids, applied in the field of active substance screening, can solve the problems that lysophospholipids and plasmalogens are difficult to remove, and free fatty acids cannot be completely removed, so as to improve brain Aβ aggregation and reduce behavioral cognitive function , the effect of broad market application prospects

Active Publication Date: 2020-08-21
OCEAN UNIV OF CHINA +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved in the present invention is that the current extraction method of plasmalogen includes organic reagent extraction and purification and enzymatic conversion of organic reagent extraction, using the above methods has several problems: the first, free fatty acids cannot be completely removed; second, It is difficult to completely separate lysophospholipids from plasmalogen, and at the same time, it has the disadvantage of solvent residue, which will limit its application range

Method used

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  • Preparation method and application of EPA/ARA type acetal phospholipid
  • Preparation method and application of EPA/ARA type acetal phospholipid
  • Preparation method and application of EPA/ARA type acetal phospholipid

Examples

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Embodiment 1

[0030] A preparation method of EPA / ARA type plasmalogen includes the following steps:

[0031] 1) Freeze and vacuum dry the mussels to obtain a powder with a fat content of 5-30%;

[0032] 2) The powder is subjected to subcritical extraction to obtain the phospholipid complex. The specific operating parameters are 1:1-1.5:1; working pressure of extraction tank: 0.3-0.8MPa; extraction temperature: 30-50°C; extraction time: 30 -60 minutes; separation tank temperature: 50-70℃,

[0033] 3) The mussel phospholipid complex is dissolved in n-hexane, the substrate concentration is controlled to 2g / mL, the amount of enzyme (phospholipase A1, Lectiase Ultra) 4%, the amount of water 10%, the reaction temperature is 45 ℃, in a heat collecting mixer (200r / min) reaction for 20h;

[0034] 4) The reaction solution is centrifuged (4000r / min) and rotary evaporated for 30 minutes to obtain enzymatically hydrolyzed phospholipids;

[0035] 5) Charge 100g of enzymatically degraded phospholipids;

[0036] 4)...

Embodiment 2

[0041] Example 2: Evaluation of biological activity of EPA / ARA plasmalogen

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Abstract

The invention belongs to the technical field of active substance screening, and relates to a preparation method and application of EPA / ARA type acetal phospholipid. The method comprises steps: continuously hydrolyzing a phospholipid complex by using PLA1 enzyme, and then extracting the EPA / ARA type acetal phospholipid from the enzymolyzed phospholipid by using a supercritical CO2 fluid extractiontechnology. The EPA / ARA type acetal phospholipid prepared by the invention has the advantages that the purity is 80% or more, compared with EPA type acetal phospholipids or ARA type acetal phospholipids, the EPA / ARA type acetal phospholipid has the advantages that the behavior cognition function of mice with the Alzheimer's disease is remarkably reduced, brain A beta aggregation and Tau protein aggregation are improved, and the EPA / ARA type acetal phospholipid can be used for preparing medicine for preventing and treating the Alzheimer's disease and other diseases and has wide market application prospects.

Description

Technical field: [0001] The invention belongs to the technical field of active substance screening, and specifically relates to a preparation method and application of an EPA / ARA type plasmalogen. Background technique: [0002] Alzheimer's disease (Alzheimer's disease, AD) is a neurodegenerative disease, is currently the most common and most destructive dementia, recognized by the World Health Organization (World Health Organization, WHO) as the world's primary public health One of the problems. The hippocampus and cortex of patients with Alzheimer’s disease will shrink. The most important neuropathological signs are the hyperphosphorylation of Tau protein and the excessive accumulation of neurofibrillary tangles and the formation of amyloid beta (Amyloidbeta, Aβ). Plaque deposition. More importantly, Alzheimer's disease has a higher mortality rate. Regrettably, the current drug treatment for AD is still in its infancy, which can provide symptom relief but will not slow down t...

Claims

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Application Information

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IPC IPC(8): C12P9/00A61K31/685A61P25/28
CPCA61K31/685A61P25/28C12P9/00Y02P20/54
Inventor 薛长湖刘炎峻徐杰姜晓明李兆杰丛培旭王玉明王静凤
Owner OCEAN UNIV OF CHINA
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