Preparation method of superparamagnetic nickel nanoparticles capable of magnetically adsorbing bacteria and spores

A magnetic adsorption and superparamagnetic technology, applied in chemical instruments and methods, inductance/transformer/magnet manufacturing, alkali metal oxides/hydroxides, etc., can solve problems such as the efficiency of adsorption not reaching expectations

Inactive Publication Date: 2020-08-18
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Metal nanoparticles with superparamagnetism can adsorb bacteria in the presence of magnets, but the adsorption efficiency is often not as expected

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The synthetic method of superparamagnetic nickel nanoparticle, concrete steps are as follows:

[0020] (1) Add NiCl to DEG containing NaOH and NaCit 2 , making it undergo thermal hydrolysis and reduction reactions. First, 15 mM NaOH was dissolved in 6 ml of DEG at 150 °C.

[0021] (2) When equipped with N 2 Add 20ml of DEG to the 250ml round bottom bottle of the inlet, condenser and mechanical stirring shaft, then add 4mM NiCl 2 and 1.7mM NaCit, in N 2 Heated to 200°C under protection and mechanically stirred at 200rpm for 30min.

[0022] (3) Then, the prepared NaOH / DEG mixture was quickly injected into the NiCl2 / NaCit / DEG phase, and the color quickly changed from greenish to black. After reacting for 2 hours, the reaction was naturally cooled to room temperature, and the magnetic analysis of superparamagnetic nickel nanoparticles (SNCNC) was performed. Before use, SNCNCs were washed twice with ethanol, three times with water, and finally preserved with ethanol. ...

Embodiment 2

[0026] The synthetic method of superparamagnetic nickel nanoparticle, concrete steps are as follows:

[0027] (1) Add NiCl to DEG containing NaOH and NaCit 2 , making it undergo thermal hydrolysis and reduction reactions. First, 15 mM NaOH was dissolved in 6 ml of DEG at 150 °C.

[0028] (2) When equipped with N 2 Add 20ml of DEG to the 250ml round bottom bottle of the inlet, condenser and mechanical stirring shaft, then add 4mM NiCl 2 and 3.4mM NaCit, in N 2 Heated to 300°C under protection and mechanically stirred at 200rpm for 30min.

[0029] (3) Then, the prepared NaOH / DEG mixture was quickly injected into the NiCl2 / NaCit / DEG phase, and the color quickly changed from greenish to black. After reacting for 2 hours, the reaction was naturally cooled to room temperature, and the magnetic analysis of superparamagnetic nickel nanoparticles (SNCNC) was performed. Before use, SNCNCs were washed twice with ethanol, three times with water, and finally preserved with ethanol. ...

Embodiment 3

[0033] The synthetic method of superparamagnetic nickel nanoparticle, concrete steps are as follows:

[0034] (1) Add NiCl to DEG containing NaOH and NaCit 2 , making it undergo thermal hydrolysis and reduction reactions. First, 30 mM NaOH was dissolved in 6 ml of DEG at 200 °C.

[0035] (2) When equipped with N 2 Add 20ml of DEG to the 250ml round bottom bottle of the inlet, condenser and mechanical stirring shaft, then add 4mM NiCl 2 and 1.7mM NaCit, in N 2 Heated to 200°C under protection and mechanically stirred at 200rpm for 30min.

[0036] (3) Then, quickly inject the prepared NaOH / DEG mixture into the NiCl 2 In the / NaCit / DEG phase, the color changes rapidly from greenish to black. After reacting for 2 hours, the reaction was naturally cooled to room temperature, and the magnetic analysis of superparamagnetic nickel nanoparticles (SNCNC) was performed. Before use, SNCNCs were washed twice with ethanol, three times with water, and finally preserved with ethanol. ...

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PUM

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Abstract

The invention discloses a preparation method of superparamagnetic nanoparticles capable of magnetically adsorbing bacteria and bacterial spores. The preparation method comprises the following specificsteps of adding NiCl2 into DEG containing NaOH and NaCit, and carrying out pyrohydrolysis and reduction reaction; rapidly injecting the prepared NaOH / DEG mixture into a NiCl2 / NaCit / DEG phase, and enabling the color to rapidly change from greenish green to black; after the reaction, cooling to room temperature, and carrying out magnetic analysis on the superparamagnetic nano nickel nanoparticles(SNCNC); culturing escherichia coli by an LB culture medium, and detecting the combination condition of SNCNC and Escherichia coli by a transmission electron microscope. The superparamagnetic nickel nanoparticles can adsorb the bacteria more efficiently so as to remove the bacteria, and can absorb the high-temperature-resistant, high-pressure-resistant and radiation-resistant bacterial spores so as to remove the spores.

Description

technical field [0001] The invention relates to a method for preparing superparamagnetic nickel nanoparticles capable of magnetically adsorbing bacteria and bacterial spores. The synthesized superparamagnetic nanoparticle can efficiently adsorb bacteria and bacterial spores, providing a new method for removing bacteria and spores. Background technique [0002] Bacteria are still an important factor causing some diseases, and bacterial infection poses a major threat to human health and food safety. At present, antibiotics are a commonly used means of sterilization, but the widespread use of antibiotics has led to the evolution of drug resistance in most bacteria, greatly reducing the efficiency of sterilization. The presence of bacterial spores is one of the important reasons for disease recurrence. Spores are a form of resistance to high temperature, radiation, and antibiotics that cannot be easily removed. Therefore, how to efficiently remove bacteria and spores is one of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): H01F41/02B01J20/02B01J20/28B01J20/30
CPCB01J20/02B01J20/28004B01J20/28009H01F41/02
Inventor 郑斌郭明明明东甘霖
Owner TIANJIN UNIV
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