Method for improving induction efficiency of neural stem cells and promoting differentiation of neurons

A neural stem cell and neuron technology, applied in the field of improving the induction efficiency of neural stem cells and promoting neuron differentiation, can solve the problems of unfavorable neural stem cell induction and neuron differentiation, troublesome research operation process, and complicated research methods, so as to improve the induction and neuron differentiation. Promote the differentiation of neurons, ensure the realization of research, and the effect of simple and convenient operation

Inactive Publication Date: 2020-08-11
ZHUJIANG HOSPITAL SOUTHERN MEDICAL UNIV
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Problems solved by technology

[0002] The traditional research methods for neural stem cell induction and neuron differentiation are relatively complicated, and the research operation pro

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  • Method for improving induction efficiency of neural stem cells and promoting differentiation of neurons

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Embodiment Construction

[0016] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention.

[0017] In describing the present invention, it should be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", " The orientation or positional relationship indicated by "outside", etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the referred device or element must have a specific orientation, so as to Specific orientation configurations and operations, therefore, are not to be construed as limitations on the invention.

[0018] refer to figure 1...

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Abstract

The present invention discloses a method for improving induction efficiency of neural stem cells and promoting differentiation of neurons. A programmed induction of SMSINS cells is as follows: using LDN193189 (Selleck S2618, 0.25 [mu]M) and SB431542 (Selleck S1067, 1 [mu]M) on the 1st and 2nd days to treat mouse embryonic fibroblasts (MEFs); using CHIR99021 (Selleck S2924, 3 [mu]M) DAPT (Selleck S2215, 5 [mu]M) and VPA (Sigma P4543, 0.5 mM) on the 3rd and 4th day; using CHIR99021 (Selleck S2924, 3 [mu]M) and DAPT (Selleck S2215, 5 [mu]M) on the 5th and 6th days; adding Shh (Peprotech 315225, 100 ng/ml) and Purmorphamine (selleck S3042, 1 [mu]M) on the 7th and 8th days to improve neural differentiation; and removing all small molecule compounds on the 9th and 10th days and conducting changeto a NSC complete culture medium. At the time, SMSINS cells can be trypsinized into single cells and can be inoculated into 24-well plates without coating PDL/L in the NSC complete culture medium; and the SMSINS cells are subjected to in vitro neuronal differentiation. The method is simple and convenient to operate, can improve induction of the neural stem cells and promote the differentiation ofthe neurons, and is beneficial to popularization and application.

Description

technical field [0001] The invention relates to the technical field of neural stem cell induction and neuron differentiation, in particular to a method for improving the induction efficiency of neural stem cells and promoting neuron differentiation. Background technique [0002] The traditional research methods for neural stem cell induction and neuron differentiation are relatively complicated, and the research operation process is cumbersome, the induction efficiency is not high, and it is not conducive to the occurrence of neural stem cell induction and neuron differentiation. To this end, we propose an applied method to increase the efficiency of neural stem cell induction and promote neuronal differentiation. Contents of the invention [0003] The purpose of the present invention is to solve the shortcomings in the prior art, and propose a method for improving the induction efficiency of neural stem cells and promoting neuron differentiation. [0004] In order to ach...

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Application Information

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IPC IPC(8): C12N5/0793
CPCC12N5/0619C12N2500/38C12N2501/01C12N2501/13C12N2533/32
Inventor 郭阳张培东段青蕊
Owner ZHUJIANG HOSPITAL SOUTHERN MEDICAL UNIV
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