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SNCG/NMP22 joint inspection colloidal gold test strip as well as preparation method and application thereof

A colloidal gold test paper and test strip technology, which is applied in the field of tumor detection, can solve the problems of increasing medical costs for patients, high maintenance costs, high costs of reagents and equipment, etc.

Pending Publication Date: 2020-08-04
BEIJING MODERN GAODA BIOTECHNOLOGY CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, chemiluminescent reagents and their supporting equipment are mainly used in large hospitals. There are also high cost of reagents and equipment, and high maintenance costs in the later stage, which will greatly increase the medical cost of patients.

Method used

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  • SNCG/NMP22 joint inspection colloidal gold test strip as well as preparation method and application thereof
  • SNCG/NMP22 joint inspection colloidal gold test strip as well as preparation method and application thereof
  • SNCG/NMP22 joint inspection colloidal gold test strip as well as preparation method and application thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0106] The present invention also provides a kind of preparation method of SNCG / NMP22 joint inspection colloidal gold test strip, described method comprises:

[0107] Preparation of colloidal gold particles;

[0108] The colloidal gold particles are combined with the first mouse anti-SNCG antibody and the first anti-NMP22 antibody to form a colloidal gold-labeled mouse anti-SNCG antibody / anti-NMP22 antibody colloidal gold conjugate;

[0109] The mouse anti-SNCG antibody / anti-NMP22 antibody colloidal gold conjugate is sprayed on the glass fiber membrane and dried to obtain a gold conjugate pad;

[0110] Prepare the detection line and the quality control line on the nitrocellulose membrane to obtain the reaction membrane, wherein the solution containing the second mouse anti-SNCG antibody, the second anti-NMP22 antibody, and the solution containing the goat anti-mouse IgG polyclonal antibody are sprayed on the SNCG detection line, NMP22 detection line and quality control line a...

Embodiment 1

[0134] In the following examples, unless otherwise specified, the substances used in the examples are commercially available products.

[0135] (1) Preparation of colloidal gold particles

[0136] Take 1.0g of chloroauric acid, equilibrate to room temperature, measure 2.0mL of purified water, put it into a glass bottle containing chloroauric acid, and blow repeatedly until the chloroauric acid is completely dissolved. Pour the dissolved auric acid into the container, rinse the glass bottle of auric acid with purified water for 5 times, and pour the washing solution back into the container. The solution was made up to 10.0 mL with purified water. Close the bottle cap tightly, shake fully for 15 minutes, mix well, and prepare 10% chloroauric acid solution.

[0137] Measure 0.3 mL of 10% chloroauric acid solution, add it into 100.0 mL of purified water, and stir until boiling while heating. Measure 0.6mL of 10% trisodium citrate solution, quickly add it to the boiling chloroau...

Embodiment 2

[0152] (1) Preparation of colloidal gold particles

[0153] The preparation steps are the same as in Example 1.

[0154] (2) Preparation of the first mouse anti-SNCG antibody and the second mouse anti-SNCG antibody

[0155] The preparation steps are the same as in Example 1.

[0156] (3) Preparation of colloidal gold-labeled mouse anti-SNCG antibody / anti-NMP22 antibody colloidal gold conjugate

[0157] The difference from Example 1 is that 2.0 mg of the first mouse anti-SNCG antibody and 2.0 mg of the first anti-NMP22 antibody prepared in step (2) were used. Replace 1.5 mL of 10 g / 100 mL sodium caseinate solution with 2.0 mL of 10 g / 100 mL sodium caseinate solution. Other reaction conditions are identical with embodiment 1.

[0158] (4) Preparation of gold conjugate pads

[0159] The difference from Example 1 is that after reconstitution, the content of the first mouse anti-SNCG antibody is 20 μg / mL, and the content of the first anti-NMP22 antibody is 20 μg / mL. Other rea...

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Abstract

The invention provides an SNCG / NMP22 joint inspection colloidal gold test strip. The test strip sequentially comprises a sample pad, a gold conjugate pad, a reaction film and an absorption pad from asample adding end, wherein the gold conjugate pad contains a first mouse anti-SNCG antibody and a first anti-NMP22 antibody, and the reaction film contains a second mouse anti-SNCG antibody and a second anti-NMP22 antibody. The SNCG / NMP22 joint inspection colloidal gold test strip prepared according to the invention not only can be used for completing conventional detection in hospitals, but alsocan be carried to home to complete detection, and compared with a colloidal gold test strip of a single antibody, the specificity and sensitivity of cancer detection can be improved, and the SNCG / NMP22 joint inspection colloidal gold test strip is more suitable for general screening of tumors.

Description

technical field [0001] The invention belongs to the field of tumor detection, in particular, the invention relates to a colloidal gold test strip for detecting tumor gamma-synuclein (SNCG) / NMP22, a preparation method and application thereof. Background technique [0002] According to the data of "China Health and Family Planning Statistical Yearbook 2015", among the number of deaths caused by diseases in my country, cancer has become the first factor. Especially in the urban population, the number of deaths caused by cancer far exceeds that of heart disease at 27%. There are more than 10,000 new cases of cancer every day in the country. Therefore, cancer has become an important killer affecting the health of our country. However, when the cancer incidence rate in my country is comparable to the world level, the mortality rate is higher than the world level (the cancer mortality rate in China is 17% higher than the global average level). In addition to the fact that the cha...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/558G01N33/574G01N33/532
CPCG01N33/68G01N33/6875G01N33/558G01N33/5748G01N33/57488G01N33/532
Inventor 唐小梅李晓楠王欢荆志强韩晓亮王建铭
Owner BEIJING MODERN GAODA BIOTECHNOLOGY CO LTD
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