Method for preparing flavonoid compound by separating flos puerariae

A technology of flavonoids and kudzu flower, which is applied in the direction of chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve the problems of time-consuming and laborious separation process, complicated components, and restricted application, so as to achieve low product cost and easy operation. simple effect

Inactive Publication Date: 2020-07-31
HEFEI UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The flavonoids in Pueraria japonica are usually separated by high-performance liquid chromatography (HPLC) or silica gel chromatography. However, due to the complex composition of flavonoids in Pueraria japonica extracts and mixed with polysaccharides, proteins and other components, Therefore, it is difficult to isolate the specific flavonoids in the kudzu extract
Combining traditional silica gel chromatography and HPLC, through repeated purif

Method used

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  • Method for preparing flavonoid compound by separating flos puerariae
  • Method for preparing flavonoid compound by separating flos puerariae
  • Method for preparing flavonoid compound by separating flos puerariae

Examples

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Embodiment 1

[0027] The specific operation steps of isolating and preparing a kind of flavonoid compound from Pueraria japonica are as follows:

[0028] (1) Preparation of zirconia

[0029] Zirconium oxychloride (ZrOCl 2 ·8H 2 O) Dissolve in deionized water (0.4 mol / L), slowly add concentrated ammonia water dropwise until the pH value is 10, continue to stir for 1 hour, and obtain a white precipitate; wash with deionized water until pH = 7, and dry in an oven at 110°C ; Calcined at 400°C for 2 hours in a muffle furnace to obtain zirconia. The prepared zirconia was analyzed by infrared spectrometer (Fig. figure 1 shown), its infrared spectrum data (KBr) is: 500-850, 1200-1460, 1460-1680 (cm -1 ); the prepared zirconia was analyzed by X crystal diffractometer (diffraction peaks as attached figure 2 shown), indicating that the prepared zirconia is a mixture of monoclinic and cubic phases.

[0030] (2) Preparation of kudzu flower crude extract

[0031] Fresh kudzu flower was dried at ...

Embodiment 2

[0043] The differences between Example 2 and Example 1 are: (1) During the preparation of zirconia, the concentration of zirconia oxychloride is 0.3mol / L, and the calcination temperature of zirconia is 300°C; (2) The crude extract is prepared The ultrasonic extraction time during the process was 90 minutes; (3) The shaking table oscillation adsorption time during the adsorption process was 6 h; (4) During the desorption process. The concentration of acetic acid is 50%, and the shaking table desorption time is 3h. Other operations and conditions are the same as in Example 1. In Example 2, 6.01 mg of 3'-hydroxyiridin-7-O-β-D-xylosyl-(1→6)-β-D-glucopyranoside was obtained, with a purity of 91% and a yield of 0.55%. .

Embodiment 3

[0045] The differences between Example 3 and Example 1 are: (1) During the preparation of zirconia, the concentration of zirconia oxychloride is 0.5mol / L, and the calcination temperature of zirconia is 200°C; The bed shaking adsorption time is 10 h; (3) The shaking bed desorption time is 9 h during the desorption process. Other operations and conditions are the same as in Example 1. In Example 3, 6.65 mg of 3'-hydroxyiridin-7-O-β-D-xylosyl-(1→6)-β-D-glucopyranoside was obtained, with a purity of 92% and a yield of 0.62%. .

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Abstract

The invention discloses a method for preparing a flavonoid compound by separating flos puerariae, and belongs to the field of preparation methods of plant natural products. The flavonoid compound is 3'-hydroxyl tectorigenin-7-O-beta-D-xylosyl-(1->6)-beta-D-glucopyranoside. According to the preparation method disclosed by the invention, zirconium oxide is used as a separation medium, and the flavonoid compound is obtained by separation through the steps of synthesizing the zirconium oxide, preparing flos puerariae crude extract, performing zirconium oxide adsorption, performing washing with distilled water and performing acetic acid desorption. The purity of the flavonoid compound reaches 90% or above, and the yield of the flavonoid compound is about 0.6%. According to the preparation method disclosed by the invention, other organic solvents are not used except acetic acid and ethyl acetate, so that the preparation method is an environment-friendly separation method; and the separationpreparation method is simple in process, free of expensive production facilities, low in product cost and suitable for industrial production.

Description

technical field [0001] The invention belongs to the field of preparation methods of plant natural products, in particular to a method for isolating a specific flavonoid compound from kudzu flower. Background technique [0002] Pueraria japonica is an important Chinese herbal medicine, which has the effects of relieving alcohol and refreshing the spleen. It is mainly used to treat symptoms such as alcohol injury, fever, polydipsia, vomiting, acid regurgitation, and lack of appetite. In recent years, studies have shown that kudzu flower also has anti-tumor and anti-stroke functions. The flavonoids in kudzu flower are the basis for its various functions, including puerarin, Nepal irisin, irisin, iris isoflavones and genistin, etc. In addition to these common known components, some new flavonoids have recently been isolated from Pueraria japonica, such as 3'-hydroxyirisanthin-7-O-β-D-xylosyl-(1→6) -β-D-glucopyranoside (its structure is shown in the figure below). The compound...

Claims

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Application Information

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IPC IPC(8): C07H1/08C07H17/07
CPCC07H1/08C07H17/07
Inventor 马意龙仲志峰冯俊杨洋商亚芳魏兆军
Owner HEFEI UNIV OF TECH
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