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Skin anti-aging ability gene detection primer combination and kit and applications of skin anti-aging ability gene detection primer combination and kit

A primer combination and primer set technology, which is applied in the direction of recombinant DNA technology, microbial measurement/inspection, biochemical equipment and methods, etc., can solve the problem that the detection does not fully meet the purpose of anti-aging gene detection, and the simultaneous detection of SNP sites cannot be realized , detection sites can not cover the detection needs and other problems, to achieve the effect of maintaining youthful vitality, flexible time, and low cost

Active Publication Date: 2020-07-28
广州市普森生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The detection aspects selected by this patent are not fully in line with the purpose of anti-aging gene detection, including other detection aspects, and the detection sites cannot cover the detection requirements
The patent (201811630156.2) discloses a method and kit for detecting anti-aging ability. By detecting 3 sites related to anti-aging, the anti-aging ability of the skin is detected by fluorescent quantitative PCR. Although the method is simple to operate, it cannot The actual need to realize the simultaneous detection of a large number of SNP sites takes a long time and the detection range is small

Method used

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  • Skin anti-aging ability gene detection primer combination and kit and applications of skin anti-aging ability gene detection primer combination and kit
  • Skin anti-aging ability gene detection primer combination and kit and applications of skin anti-aging ability gene detection primer combination and kit
  • Skin anti-aging ability gene detection primer combination and kit and applications of skin anti-aging ability gene detection primer combination and kit

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Experimental program
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Effect test

Embodiment 1

[0089] Step 1: Synthesize multiplex PCR amplification primer sets and single base extension primer sets for desired SNP sites.

[0090] Step 2: Extract DNA from human oral swab samples and perform PCR amplification.

[0091] Step 3: Using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) to detect the DNA of the sample and determine the genotype of the SNP site of the sample.

[0092] Step 4: Comprehensively evaluate the genetic information of the skin's anti-aging ability based on the genotype detection results of the SNP sites, and give an interpretation of the results.

[0093] Table 2 Multiplex PCR amplification primers and single base extension primer sequences

[0094]

[0095]

[0096]

Embodiment 2

[0098] In order to verify the accuracy and practicability of the multiplex PCR amplification primer set and single base extension primer set design for detecting the SNP site of the skin anti-aging ability gene of the present invention, 30 oral swab samples were collected in this embodiment. Detection, the specific content is as follows:

[0099] 1. Primer design:

[0100] According to the SNP site sequence information, use the design software Assay Design Suite V2.0 of Agena Company to design multiple PCR amplification primers and single-base extension primers for the SNP sites to be tested, and multiplex PCR amplification primers for 21 SNP sites in 17 genes Amplification primer sets and single base extension primer sets were designed into one reaction (WELL) and validated with UCSC GenomeBioinformatics.

[0101] The result of the quality value of the primer combination of the design software shows that the quality of the primer combination published by the present inventio...

Embodiment 3

[0160] Using multiplex PCR amplification primer set and single base extension primer set designed by the present invention and ordinary multiplex PCR amplification primer set and single base extension primer set (Table 13) to detect buccal swab DNA samples respectively, sample extraction and detection The specific implementation methods and steps are as above-mentioned Example 1 and Example 2, and the result data are shown in Table 14.

[0161] Table 13 Common Multiplex PCR Amplification Primer and Single Base Extension Primer Sequence

[0162]

[0163]

[0164]

[0165] The test result data of this comparison experiment shows that the multiplex PCR amplification primer set and single base extension primer set designed by the present invention are more suitable for the SNP site of skin anti-aging ability gene than ordinary multiplex PCR amplification primer set and single base extension primer set Detection (Table 14). The positive detection rate of the multiple PCR ...

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Abstract

The present invention relates to the technical field of genotyping and particularly to a skin anti-aging ability gene detection primer combination and a kit and applications of the skin anti-aging ability gene detection primer combination and kit. A pair of multiple PCR amplification primers and a single base extension primer are separately designed for each SNP site. All sites are designed into one reaction and can be detected simultaneously. The multiple PCR amplification primer set and the single base extension primer set are used for MassARRAY nucleic acid mass spectrometry analysis systemdetection. The system can be used for rapid analysis of nucleic acid samples with high sensitivity and high accuracy, and a detection accuracy rate is larger than or equal to 99.9%. An SNP typing method for efficiently detecting skin anti-aging ability genes is established, can complete molecular biology genotyping of multiple SNP sites at the same time, has good detection sensitivity, high accuracy, low cost and high practicality, can be used to detect genetic factors that affect skin anti-aging ability, comprehensively evaluates the skin anti-aging ability, and helps promote development ofindividualized services in the feature-beautifying industry.

Description

technical field [0001] The invention relates to the technical field of genotyping, in particular to a combination of skin anti-aging gene detection primers, a kit and applications thereof. Background technique [0002] At this stage, people's demand for skin care has reached an unprecedented height, and the spending on skin care is also increasing, and the proportion of anti-aging in skin care demand is gradually increasing. Comprehensive anti-aging care has become an important part of skin care. According to the research, the share of anti-aging skin care products in the Chinese population has reached 23%, and it has become the largest category of skin care products. Among the needs of whitening, freckle removal, moisturizing, anti-aging, etc., the mention rate of anti-aging is as high as 83%, and the survey results show that among the most concerned and popular beauty and skin care services and products for women, the mention rate of anti-aging is as high as 72.2% , rank...

Claims

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Application Information

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IPC IPC(8): C12Q1/6876C12Q1/6858C12N15/11
CPCC12Q1/6858C12Q1/6876C12Q2600/156C12Q2600/16C12Q2531/113C12Q2537/143C12Q2533/101C12Q2565/627
Inventor 李文郭之彬张欣鑫廖俊杰
Owner 广州市普森生物科技有限公司
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