Injection device for hair follicle stem cell culture and using method

Abstract

The invention relates to the technical field of hair follicle stem cell culture, and specifically relates to an injection device for hair follicle stem cell culture and a using method. The device comprises a shell body, a culture dish, a liquid-injection structure, an injection structure, an auxiliary structure, a protective structure and a fixed structure. The injection structure arranged on theshell body facilitates the injection of stem cells into the interior of the culture dish without contacting with air, and the culture dish is stably installed inside the shell body through the cooperation use of the protective structure, which is conducive to the protection of stem cell culture while injecting stem cells, and preventing stem cells from contacting with air. Through the linkage of the injection structure and the liquid-injection structure, after the stem cells are injected into the culture dish by the injection structure, nutrition solution is injected by the liquid-injection structure from the injection structure into the culture dish for the growth of stem cells, the interior of the injection structure is washed, which makes the interior of the injection structure clean while some attached stem cells are flushed into the interior of the culture dish, and at the same time, the nutrition solution enters the interior of the culture dish easily and smoothly from the injection structure.

Description

technical field [0001] The invention relates to the technical field of hair follicle stem cell culture, in particular to an injection device and a use method for hair follicle stem cell culture. Background technique [0002] Hair follicle stem cells are a type in the bulge of the outer root sheath of human hair follicles. Hair follicle stem cells belong to adult stem cells, which are in a quiescent state in vivo, and show amazing proliferation ability under in vitro culture. Hair follicle stem cells have multi-directional differentiation potential. They can differentiate into epidermis, hair follicles, and sebaceous glands, and participate in the process of skin wound healing. Stem cells are in hair follicles, and like other adult stem cells, have the characteristics of slow cycle, undifferentiated, self-renewal and strong proliferation ability in vitro. [0003] The traditional hair follicle stem cells are cultivated by injecting the stem cell culture solution into the cul...

AI Technical Summary

Problems solved by technology

[0003] The traditional hair follicle stem cells are cultivated by injecting the stem cell culture solution into the culture dish one by one, which reduces the work efficiency. At the same time, the hair follicle stem cells are in contact with the air for a long time during the injection culture process, so that the hair follicle stem cell culture solution is easily mixed with other substances such as bacteria, resulting in Sterilization of stem cells takes a long time, making it difficult for stem cells to survive and affecting the survival of stem cells

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