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Gene detection primer combination for acne resistance of skin and application of gene detection primer combination

A primer combination and primer set technology, applied in recombinant DNA technology, microbial determination/inspection, biochemical equipment and methods, etc., can solve the problems of low detection specificity, small detection range, complicated operation, etc., to avoid crossover Contamination, time flexibility, high sensitivity effects

Active Publication Date: 2020-06-23
广州市普森生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The patent (201811143772.5) discloses a method for rapid detection of hereditary skin anti-acne genes. Two gene loci rs7531806 and rs747150 related to anti-acne are detected by the HRM method. This method can detect the difference of a single base, but its The detection range is small and the operation is relatively complicated, which cannot meet the needs of detecting more than two gene loci related to skin anti-acne ability
The patent (201810839384.4) discloses a skin-related gene locus library and its construction method and application, aiming at the detection of 5 anti-acne genes, including: sebaceous gland secretion-related gene BCMO1, microbial susceptibility-related genes SELL, DDB2, inflammatory factor secretion The detection of related genes IL-6 and TNF-alpha, the detection method of this patent can accurately determine the cause of skin problems, but there are many classifications, the detection specificity is not high, and the operation is relatively complicated

Method used

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  • Gene detection primer combination for acne resistance of skin and application of gene detection primer combination
  • Gene detection primer combination for acne resistance of skin and application of gene detection primer combination
  • Gene detection primer combination for acne resistance of skin and application of gene detection primer combination

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Experimental program
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Effect test

Embodiment 1

[0073] A skin anti-acne ability gene detection primer combination and its application, including multiple PCR amplification primer sets and single base extension primer sets for detecting 8 SNP sites of 5 skin anti-acne ability genes in the human genome (Table 2) . A pair of multiplex PCR amplification primers and a single-base extension primer are designed for each SNP site, and all sites are designed into one reaction for simultaneous detection. The multiple PCR amplification primer set and the single base extension primer set are used in the MassARRAY nucleic acid mass spectrometry analysis system, which can be used for rapid analysis of nucleic acid samples with high sensitivity and high accuracy, and the detection accuracy rate is ≥99.9%. The present invention establishes an efficient SNP typing method for comprehensively evaluating skin anti-acne ability genes, which can complete the molecular biology genotyping of multiple SNP sites at the same time, with good detection...

Embodiment 2

[0081] Example 2 Sample Detection

[0082] In order to verify the accuracy and practicability of the multiplex PCR amplification primer set and single base extension primer set design for detecting the SNP site of the skin anti-acne ability gene of the present invention, 30 oral swab samples were collected in this embodiment. Detection, the specific content is as follows:

[0083] 1. Primer design:

[0084] According to the SNP site sequence information, use the design software Assay Design Suite V2.0 of Agena Company to design multiple PCR amplification primers and single-base extension primers for the SNP sites to be tested, and multiplex PCR for 8 SNP sites of 5 genes Amplification primer sets and single base extension primer sets were designed into one reaction (WELL) and validated with UCSC GenomeBioinformatics.

[0085] The result of the quality value of the primer combination of the design software shows that the quality of the primer combination published by the pres...

Embodiment 3

[0146] Example 3 Primer Comparison

[0147] Using multiplex PCR amplification primer set and single base extension primer set designed by the present invention and ordinary multiplex PCR amplification primer set and single base extension primer set (Table 13) to detect buccal swab DNA samples respectively, sample extraction and detection The specific implementation methods and steps are as above-mentioned Example 1 and Example 2, and the result data are shown in Table 14.

[0148] Table 13 Common Multiplex PCR Amplification Primer and Single Base Extension Primer Sequence

[0149]

[0150]

[0151] The test result data of this comparison experiment shows that the multiplex PCR amplification primer set and single base extension primer set designed by the present invention are more suitable for the SNP site of skin anti-acne ability gene than ordinary multiplex PCR amplification primer set and single base extension primer set Detection (Table 14). The positive detection ...

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Abstract

The invention relates to the technical field of genotyping, and discloses a gene detection primer combination for acne resistance of a skin and an application of the gene detection primer combination.The gene detection primer combination comprises a multiplex PCR amplification primer group and a single base extension primer group which are used for detecting eight SNP loci of five acne resistancegenes of a human genome. Each SNP locus is designed with a pair of multiplex PCR amplification primers and a single base extension primer, and all the sites are designed into a reaction and can be detected simultaneously. According to the invention, an SNP typing method for efficiently detecting the anti-acne gene of the skin is established, and molecular biological genotyping of a plurality of SNP loci can be completed at the same time. The detection sensitivity is good, accuracy is high, cost is low, and practicability is high. The SNP typing method can be used for detecting genetic factorsinfluencing acne resistance of the skin and comprehensively evaluating acne resistance of the skin, and promotes development of individual service in the beauty industry.

Description

technical field [0001] The invention relates to the technical field of genotyping, in particular to a combination of skin anti-acne ability gene detection primers and an application thereof. Background technique [0002] Acne, a professional term for acne, is a very common chronic inflammatory skin disease of the pilosebaceous glands, mainly affecting adolescents and young adults, with a prevalence rate as high as 40% to 80%, mainly manifested as facial acne and other skin problems, Severe cases or informal treatment may leave scars, affecting the patient's appearance and mental health. According to the survey data of some acne patients in my country, it shows that 58.8% of patients do not have a correct understanding of the occurrence and prevention of acne, and their self-protection is not enough, which directly affects the treatment effect of acne. Only 41.2% of acne patients choose to go to the hospital Seek professional treatment and prevention guidance. As people's de...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12Q1/6872C12Q1/686C12N15/11
CPCC12Q1/686C12Q1/6872C12Q1/6883C12Q2600/148C12Q2600/156C12Q2600/16C12Q2537/143
Inventor 李文郭之彬张欣鑫廖俊杰
Owner 广州市普森生物科技有限公司
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