Method for rapidly detecting bacteriophage in escherichia coli fermentation seed solution

Abstract

The invention relates to a method for rapidly detecting bacteriophage in an escherichia coli fermentation seed solution. The method is characterized by comprising the following steps of: culturing anescherichia coli primary fermentation seed solution overnight, centrifuging 1ml of the solution, taking the supernatant, detecting the bacteriophage by a double-layer plate method, culturing at 37 DEGC for 12-20 hours, and observing whether bacteriophage plaque appears. The method for rapidly detecting the bacteriophage in the escherichia coli fermentation seed solution has the beneficial effectsthat the possibility that the seed is infected with the bacteriophage is eliminated before production, and smooth production is ensured. A method for rapidly detecting the bacteriophage in the seed solution for fermentation is established, whether the seed solution contains the bacteriophage is rapidly detected, and subsequent fermentation failure is avoided.

Description

technical field [0001] The invention relates to the technical field of detection, in particular to a rapid detection method for bacteriophage in Escherichia coli fermentation seed liquid. Background technique [0002] Escherichia coli is the most widely used strain in industrial production, used to produce various products such as proteins, nucleic acids and small peptides. During the growth and development of Escherichia coli, various nutrients are required, which are usually provided by the components in the medium. In the prior art, before production, the possibility of seed infection with phage cannot be ruled out, and smooth production cannot be ensured. Contents of the invention [0003] The technical problem to be solved by the present invention is to provide a rapid detection method for phage in Escherichia coli fermented seed liquid. Before production, the possibility of seed infection with phage cannot be ruled out, and smooth production cannot be ensured. [0...

AI Technical Summary

Problems solved by technology

In the prior art, the possibility of seed infection with phage cannot be ruled out before production, and the smooth progress of production cannot be ensured

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