Device and method for quickly detecting environmental phage

Abstract

The invention relates to a device and a method for quickly detecting environmental phage. The device is characterized in that the top of a stainless steel tank body is bound by eight layers of gauze;a double-layer flat panel coated with sensitive fungus is placed inside the stainless steel tank body, and the air is exhausted from the bottom of the stainless steel tank body through a thin-tube vacuum pump; the air is introduced into the stainless steel tank body from the top of the stainless steel tank body and penetrates through the flat panel, and the phage of the air is adsorbed on the flatpanel; after 2 hours, the flat panel is taken out, an upper cover covers the stainless steel tank, and then the stainless steel tank is placed into a 37-degree culturing box, and whether bacteriophage plaque is produced or not needs to be checked after 6 hours. The device and the method disclosed by the invention have the advantages that a method for quickly detecting the phage of seed liquid forfermenting bacillus coli is provided; before production, the possibility of infecting the phage by the seeds is ruled out, so that production can be successfully carried out. The method for detectingthe phage of the seed liquid for fermentation is built, whether the phage exists in the seed liquid is quickly detected, and subsequent fermentation failure is avoided.

Description

technical field [0001] The invention relates to the technical field of detection, in particular to a device and method for rapidly detecting environmental phages. Background technique [0002] Escherichia coli is the most widely used strain in industrial production, used to produce various products such as proteins, nucleic acids and small peptides. During the growth and development of Escherichia coli, various nutrients are required, which are usually provided by the components in the medium. In the prior art, before production, the possibility of seed infection with phages cannot be ruled out, and the smooth progress of production cannot be ensured, especially when phages exist in the production workshop, and fermentation failure cannot be avoided. Contents of the invention [0003] The technical problem to be solved by the present invention is to provide a method for establishing a phage in the production workshop environment, and a device and method for rapidly detect...

AI Technical Summary

Problems solved by technology

In the prior art, before production, the possibility of seed infection with phage cannot be ruled out, and the smooth progress of production cannot be ensured, especially when phage exists in the production workshop, fermentation failure cannot be avoided.

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