Surface active complex enzyme, bionic peat humic acid as well as preparation method and application of surface active complex enzyme

A peat humic acid and surface active technology, applied in the fields of botanical equipment and methods, biochemical equipment and methods, microorganism-based methods, etc., can solve the problems of easy pollution, poor operating environment, long composting fermentation time, etc. Crop quality, reducing the effect of external energy supply

Active Publication Date: 2020-06-19
上海臻衍生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem actually solved by the present invention is to overcome the long fermentation time of compost in the prior art, the occurrence of peculiar smell, poor operating environment, and the product is affected by raw materials, high salt content, easy to pollute, etc. Defects, a surface active compound enzyme, bionic peat humic acid and its preparation method and application are provided

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] 1. Preparation of surface active compound enzyme

[0047] Inoculate the mixed strains in the mixed medium for fermentation and culture;

[0048] In the mixed strains, the ratio of yeast, rhizobium, Penicillium grassa, plant lactic acid bacteria, and Bacillus subtilis is 15:5:20:25:40; in the mixed medium, the mass ratio of PDA medium and MRS medium is 1: 2. The inoculum amount of the mixed strains in the mixed medium is 0.05%; the fermentation time is 58 hours, the fermentation temperature is 36°C, and the enzyme activity index of the surface active compound enzyme is 215U / g. In the surface-active compound enzyme, the mass ratio of cellulase, hemicellulase, protease, hydrolase, oxidoreductase and esterase is 10:5:20:30:30:15 (each in the surface-active compound enzyme The specific enzymes were separated, weighed separately, and calculated).

[0049] The surface tension of the surface active compound enzyme was measured by K100 surface tensiometer from German Kruss Com...

Embodiment 2

[0058] 1. Preparation of surface active compound enzyme

[0059] Inoculate the mixed strains in the mixed medium for fermentation and culture;

[0060] In the mixed strains, the ratio of yeast, rhizobium, Penicillium grassa, plant lactic acid bacteria, and Bacillus subtilis is 10:5:20:25:35; in the mixed medium, the mass ratio of PDA medium and MRS medium is 1: 2.5; the inoculum amount of the mixed strains in the mixed medium is 0.03%; the fermentation time is 65 hours, the fermentation temperature is 35°C, and the enzyme activity index of the surface active compound enzyme is 177U / g. In the surface active compound enzyme, the mass ratio of cellulase, hemicellulase, protease, hydrolase, oxidoreductase and esterase is 10:5:20:30:30:15.

[0061] 2. Preparation of biomimetic peat humic acid

[0062] Enzymolysis can be carried out in the crop stalks and water medium containing the above-mentioned surface active compound enzyme. The enzymolysis temperature is 30°C, and the enzym...

Embodiment 3

[0067] 1. Preparation of surface active compound enzyme

[0068] Inoculate the mixed strains in the mixed medium for fermentation and culture;

[0069] In the mixed strains, the ratio of yeast, rhizobium, Penicillium grassa, plant lactic acid bacteria, and Bacillus subtilis is 10:5:15:25:35; in the mixed medium, the mass ratio of PDA medium and MRS medium is 1: 1.5; the inoculum amount of the mixed strains in the mixed medium is 0.06%; the fermentation time is 72 hours, the fermentation temperature is 37°C, and the enzyme activity index of the surface active compound enzyme is 172U / g. In the surface active compound enzyme, the mass ratio of cellulase, hemicellulase, protease, hydrolase, oxidoreductase and esterase is 10:5:20:30:30:15.

[0070] 2. Preparation of biomimetic peat humic acid

[0071] Enzymolysis can be carried out in the crop stalks and water medium containing the above-mentioned surface active compound enzyme. The enzymolysis temperature is 30°C, and the enzym...

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Abstract

The invention discloses a surface active complex enzyme, bionic peat humic acid as well as a preparation method and application of the surface active complex enzyme. The preparation method of the surface active complex enzyme comprises the following steps: inoculating a mixed culture medium with a mixed strain for fermentation culture, wherein the mixed strain comprises yeast, rhizobium, penicillium, lactobacillus plantarum and bacillus subtilis, and the mixed culture medium is a mixture of PDA culture medium and an MRS culture medium. The surface active complex enzyme can line a reaction interface under a micro water or hydrophilic condition, remove harmful bacteria, reduce external energy supply and rapidly oxidize or ectopically condense straw lignin into humic acid. The bionic peat humic acid can be applied to rice, vegetables, flower seedling culture and cultivation media, and can also be applied to removal of rice field moss spore coats, humic acid bio-organic fertilizer, soil improvement remediation agents, biological stimulin, Chinese herbal medicines in soiless culture planting as well as rapid fermentation for returning to the field.

Description

technical field [0001] The invention relates to a surface-active composite enzyme, bionic peat humic acid and a preparation method and application thereof. Background technique [0002] Peat is a natural swamp product (also known as peat or peat) formed after thousands of years. It is the coal with the lowest degree of coalification, and it is also the most primitive state of coal. It is sterile, non-toxic, and non-polluting. Good ventilation performance. It is light in weight, retains water and fertilizer, is beneficial to microbial activities, enhances biological performance, and is rich in nutrients. It is not only a seedling raising substrate, a cultivation substrate, but also a good soil conditioner, and contains high organic matter, humic acid and nutrients. However, peat is a non-renewable resource, and some mining activities have caused great damage to the environment. [0003] Peat humic acid is a term of peat geology, which refers to the organic mixed acid in pea...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12N1/20C12N1/14C12N9/00C05G3/80A01G24/22A01G24/30C12R1/41C12R1/80C12R1/125C12R1/25
CPCC12N1/16C12N1/20C12N1/14C12N9/00A01G24/22A01G24/30Y02W30/40
Inventor 周霞萍
Owner 上海臻衍生物科技有限公司
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